Zhang, X.-Y. — Page 2 | Search bioRxiv

bioRxiv2022

Application and Evaluation of Flipped Teaching Based on Video Conference in Standardized Training for Internal Medicine Residents during Vacation

BackgroundInfectious disease training was a necessary part of standardized training for internal medicine residents, and a designated hospital by the health administration department provided infectious diseases training for residents in those hospitals that did not meet the training standards for infectious diseases in the region. However, due to holidays, coordination of training dates among hospitals, and vacation of other reasons, the actual training time in the Department of Infectious Diseases might be insufficient. ObjectiveI aimed to explore Flipped Teaching with Video Conference as the carrier in infectious disease training for internal medicine residents, to make up for the lack of actual training time of the Department of Infectious Diseases for those residents caused by vacation, and to ensure the smooth implementation and quality assurance of infectious disease training for those residents. MethodsVertical management mode was adopted, management team and lecturer team were established, and training program and teaching implementation were formulated. Flipped Teaching based on Video Conference was carried out for internal medicine residents of dispatched hospitals who planned to participate in infectious diseases training of the designated hospital in April. The quantitative analysis was applied to this teaching evaluation, and the evaluation indexes were included into statistical analysis to evaluate the effect of the teaching model. ResultsAll 19-member internal medicine residents participated in the Flipped Teach based on Video Conference from April 1 to 4, of which 12 residents were scheduled to participate in infectious diseases training from March 1 to April 30, and 7 residents were scheduled to participate in infectious diseases training from April 1 to May 31. A management team of 6 internal medicine residents was built, and a group of 12 lecturers were composed of 12 those residents who were scheduled to receive infectious diseases training in the designated hospital from March 1 to April 30. According to the requirements of training diseases in the Department of Infectious Diseases, 12 training contents were selected to carry out teaching, and the implementation rate of teaching plan was over 90%. A total of 197 feedback questionnaires were collected. The feedback that the teaching quality was "good" and "very good" accounted for more than 96%, and the attendance rate of the whole teaching process reached more than 94%. About this teaching, 6 internal medicine residents put forward 18 suggestions of "Improvement suggestions", accounting for 9.1%; and 11 internal medicine residents gave 110 suggestions of "Praise highlights", accounting for 55.8%. The overall evaluation feedback of flipped teaching was good, P<0.001. ConclusionFlipped Teach based on Video Conference was generally effective in carrying out for internal medicine residents participating in the infectious diseases training, and it could be used as a supplementary training method for standardized training of internal medicine residents to make up for the shortage of actual training period in a certain stage.

scientific communication and education↗

bioRxiv2021

Mapping brain-wide excitatory projectome of primate prefrontal cortex at submicron resolution: relevance to diffusion tractography

Resolving trajectories of axonal pathways in the primate prefrontal cortex remains crucial to gain insights into higher-order processes of cognition and emotion, which requires a comprehensive map of axonal projections linking demarcated subdivisions of prefrontal cortex and the rest of brain. Here we report a mesoscale excitatory projectome issued from the ventrolateral prefrontal cortex (vlPFC) to the entire macaque brain by using viral-based genetic axonal tracing in tandem with high-throughput serial two-photon tomography, which demonstrated prominent monosynaptic projections to other prefrontal areas, temporal, limbic and subcortical areas, relatively weak projections to parietal and insular cortices but no projections directly to the occipital lobe. In a common 3D space, we quantitatively validated an atlas of diffusion tractography-derived vlPFC connections with correlative enhanced green fluorescent protein-labelled axonal tracing, and observed generally good agreement except a major difference in the posterior projections of inferior fronto-occipital fasciculus. These findings raise an intriguing question as to how neural information passes along long-range association fiber bundles in macaque brains, and call for the caution of using diffusion tractography to map the wiring diagram of brain circuits.

neuroscience↗

bioRxiv2019

Whole genome sequencing of 358 brown planthoppers uncovers the landscape of their migration and dispersal worldwide

The brown planthopper (BPH), Nilaparvata lugens, is a serious migratory rice pest, which is distributed in the broad area of the tropical and temperate Asian-Pacific region. However, we know little about key aspects regarding its evolution such as how they diverged and dispersed worldwide. By resequencing and analyzing 358 BPH genomes from 92 populations across the world, we uncover the genetic relationships among their worldwide populations and the history of their global dispersal. We recovered five genetic groups representing the major population structures. Of these, Australian BPHs were shown large genetic divergence with Asian BPHs; two distinct groups have formed in South and Southeast/East Asia that show strong genetic admixture in the southwest border regions of China and west Thailand with Myanmar; two local populations in Bangladesh and Fujian province of China, respectively, unexpectedly separated with surrounding populations. We also find the genetic similarity and closely phylogenetic relationships between majority of East Asian BPHs and Indo-china peninsula BPHs, indicating that Southeast Asia mainland is the major insect sources and overwintering sites for East Asia. Our study provides important molecular evidence to address BPH evolution and other key aspects of its biology such as insecticides resistance and rice varieties virulence.

evolutionary biology↗