Yuzwa, S. A. | Search bioRxiv

bioRxiv2025

RARS1 integration into the multisynthetase complex is crucial for mammalian brain development

Aminoacyl tRNA synthetases occupy a central role in protein synthesis by charging tRNAs with their cognate amino acid. Eight aminoacyl-tRNA synthetases with nine enzymatic activities form a large protein complex but the breadth of functions mediated by the multisynthetase complex remains elusive. Neurological disorders have been associated with mutations within the domain tethering Arginyl-tRNA synthetase (RARS1) to the multisynthetase complex, which offers an interesting bridge between protein synthesis and neurodevelopment. To interrogate this connection, we developed a mouse model where RARS1 is excluded from the multisynthetase complex in the forebrain. We observed profound disruptions in neural development as attested by drastically reduced forebrain size and behavioral deficits. At the molecular level, neurodevelopmental and ribosomal genes were dysregulated. Finally, the subcellular localization of RARS1 and its colocalization with other translational machinery components were perturbed. Altogether, our results reveal the necessity of RARS1 integration in the multisynthetase complex for proper neurodevelopment.

molecular biology↗

bioRxiv2025

Spatially resolved transcriptomics identifies intercellular signaling post-ischemic stroke that controls neural stem cell proliferation

Stroke is the second leading cause of death and disability worldwide. Ischemic stroke mobilizes adult neural stem cells (NSCs) out of the quiescent state. The multifaceted responses of endogenous NSCs to ischemic stroke involve proliferation, migration, and differentiation. Hence one strategy which could be leveraged for recovery after ischemic stroke is the intrinsic mechanism of endogenous NSC mobilization. However, the survival rate of recruited endogenous NSCs is low. Moreover, the intercellular signals that activate NSCs after ischemic stroke are poorly understood. We hypothesized that after stroke, cells located in the cerebral lesion send signals to the NSC niche to initiate the regenerative response. To test this hypothesis, we used CellChat to computationally infer the cell-cell communication between the ischemic infarct region and ventricular-subventricular zone (V-SVZ) NSC niche from spatial gene expression profiles. We identified ligand-receptor pairs and signaling pathways involved in the signal transduction events at 2, 10, and 21 days after stroke. Out of several candidate genes of interest we identified, here we reported the regulatory function of galectin-9 on the proliferation of NSCs. Our present work portrays galectin-9 as a checkpoint signaling molecule that guards the responses of NSCs under physiological conditions and potentially during the recovery phase post-ischemic stroke. We suggest that TIM-3 mediates the inhibitory effect of galectin-9 on NSC proliferation and propose a working hypothesis that the stroke-induced proinflammatory factors stimulate the Toll-like receptor 4 (TLR4) on ependymal cells and result in the increased secretion of galectin-9, which in turn modulates neighboring NSCs. Our study paves the way for potential therapeutic approaches which leverage the TLR4 and galectin-9/TIM3 signaling pathways.

neuroscience↗