Biology subjects

Yoo, C.

Publications and source records attributed to Yoo, C..

4 recordsLinked to original sources

bioRxiv2024

Bilateral integration in somatosensory cortex is controlled by behavioral relevance

Sensory perception naturally requires processing stimuli from both sides of the body. Yet, how neurons bind stimulus features across the hemispheres to create a unified perceptual experience remains unknown. To address this question, we performed large-scale recordings from neurons in both somatosensory cortices (S1) while mice shared information between their hemispheres and discriminated between two categories of bilateral stimuli. When expert mice touched stimuli associated with reward, they moved their whiskers with greater bilateral symmetry. During this period, synchronous spiking and enhanced spike-field coupling emerged between the hemispheres. This coordinated activity was absent in stimulus-matched naive animals, indicating that interhemispheric (IH) binding was controlled by a goal-directed, internal process. In S1 neurons, the addition of ipsilateral touch primarily facilitated the contralateral, principal whisker response. This facilitation primarily emerged for reward-associated stimuli and was lost on trials where expert mice failed to respond. Taken together, these results reveal a novel state-dependent logic underlying bilateral integration in S1, where stimulus binding and facilitation are controlled by behavioral relevance.

neuroscience↗

bioRxiv2024

Assessment of Remediation Through Measuring Nuclear Respiratory Factor 1 (nrf1) Environmental Stressor Transcriptomic Gene Signatures

Withdrawal StatementThis manuscript has been withdrawn by the authors as it was submitted and made public without the full consent of all the authors. Therefore, the authors do not wish this work to be cited as reference for the project. If you have any questions, please contact the corresponding author.

genomics↗

bioRxiv2023

Optimization of Shoot Regeneration and Application of CRISPR/Cas9 Gene Editing to Cultivated Strawberry

Efficient methods of plant transformation and tissue culture are essential to CRISPR/Cas-based gene editing of crops, but neither is well established in cultivated octoploid strawberry (F xananassa). In the present study, a method for shoot regeneration was established and optimized for two strawberry cultivars commercially grown in Florida, Sweet Sensation(R) Florida 127 FL127) and Florida Brilliance (FB). Runner segments at the tip, node, and petiole obtained from greenhouse-grown plants were used as explants for comparisons of shoot regeneration rate. FL127 showed the highest frequency of shoot regeneration to a basal Murashige and Skoog media (MS) containing 1 mg{middle dot}L-1 of TDZ, 0.05 mg{middle dot}L-1 of BA, and 0.05 mg{middle dot}L-1 of 2,4-D, while FB showed the best response to a lower concentration of BA (0.01 mg{middle dot}L-1) in the same media type. The average conversion frequencies of somatic embryos into shoot regenerations from the runner tips (RT) were 42.8% in FL127 and 56.9% in FB, respectively, with RT being the most prolific in shoot generation for both cultivars. Using these optimized tissue conditions, Agrobacterium-mediated CRISPR/Cas9 gene editing was conducted to evaluate the efficiency of transformation and knockout mutations in the phytoene desaturase (FaPDS) gene of FL127. A total of 234 explants treated with Agrobacterium resulted in an 80.3% regeneration efficiency, with 13.3% of regenerated plants exhibiting partial or complete albino phenotypes. Amplicon sequencing of edited progeny revealed substitutions, insertions, and deletions at the gRNA target sites or flanking regions of all FaPDS homoeologous copies. Our results provide effective methods of tissue culture and transformation for the efficient application of CRISPR-mediated gene editing in cultivated strawberry.

plant biology↗

bioRxiv2023

Topographical similarity of cortical thickness represents generalized anxiety symptoms in adolescence

Generalized anxiety disorder (GAD) is a common condition characterized by excessive, uncontrollable worry. Despite increasing efforts to identify the neural underpinnings of GAD, neuroimaging research using cortical thickness have yielded largely inconsistent results. To address this, we adopted an inter-subject representational similarity analysis framework and utilized a sample of 120 adolescents (13 to 18 years of age) from the Healthy Brain Network dataset. We found greater topographical resemblance among participants with heightened generalized anxiety symptoms in the left caudal anterior cingulate and pericalcarine cortex. Such associations were not observed when including a group of younger participants (11 to 12 years of age), highlighting the importance of age range selection when considering the link between cortical thickness and anxiety. Our findings reveal a novel cortical thickness topography that represents generalized anxiety in adolescents, which is embedded within the shared geometries between generalized anxiety symptoms and cortical thickness.

neuroscience↗