Biology subjects

Yasin, J. K.

Publications and source records attributed to Yasin, J. K..

3 recordsLinked to original sources

bioRxiv2018

Novel, economically important semi-dwarf and early mutants: Selection and development from Improved White Ponni Rice (Oryza sativa L.)

Rice variety, Improved White Ponni is a medium duration crop, but highly susceptible to lodging impacting maximum yield losses. The present investigation aimed to identify early and early semi-dwarf mutants in Improved White Ponni by inducing variations using gamma rays without altering its native grain quality traits. Seeds were treated with various doses of after fixation of the LD50 value of gamma radiation and reported that most of the traits exhibit variations in the mutants at various levels of irradiation. The selection for earliness and dwarf plant height was imposed in M2 and it was confirmed by evaluation of M3 generation. Apart from semi-dwarf early mutants, high tillering habit, narrow rolled leaf, upper albino leaf and grassy stunt and extreme dwarf mutants were also identified. Characterization of mutants using already reported genic and linked microsatellite markers associated with semi-dwarfism and earliness resulted that PIC value ranges between 0.037 and 0.949 with an average of 0.382. Single marker analysis revealed that RM302 and RM310 on chromosome 1 and 8 had exhibited an association with the traits plant height, culm and internodal lengths. Of these gene-specific markers, GA20Oxi_1 and GA20Oxi_2 have shown polymorphism among mutants. GA20Oxi_2 showed null alleles in the dwarf mutants and this clearly emphasized that there are some base deletions exists in the region of exon 2 of sd1 region. GA3 response study shown that identified mutants were GA3 responsive except IWP 11-2, IWP 48-2, IWP 50-11 and IWP 33-2 which showed very low responsive. Agar plate assay revealed that, IWP 50-11, IWP 33-2, IWP 43-1, IWP 47-2 and IWP 18-1 had low production of - amylase. Scanning electron microscope examination on confirmed mutants exhibited larger cell size and a lesser number of cells per unit area than the wild-type which shows that these mutants are defective in GA mediated pathway.

plant biology↗

bioRxiv2018

Carbonic anhydrase network of genes trigger cytosolic pH enabling differentiation from quiescence

BackgroundCarbonic anhydrase regulates various cellular processes. Intracellular pH flux impacted by carbonic anhydrase alters the enzymes allosteric active site which effects several downstream cellular processes. Earlier, we reported that, the catalytic activity of carbonic anhydrase is independent, but direction of catalysis is affected by cellular pH level. On the other hand carbonic anhydrase alters the cytosolic pH level to facilitate allosteric phosphorylation of proteins which further leads to cellular differentiation through a process being regulated by ncRNAs.\n\nResultsThis study illustrates various ways of cell differentiation/ organ development regulation via carbonic anhydrase interacting network of proteins involved in various cellular processes. It is involved in protein degradation process of other proteins like RPT, 26S proteasome, AT3G15120 and its variant producing ncRNA, etc. Carbonic anhydrase indirectly involved in signaling process along with MAPK in providing innate resistance against biotic and abiotic stresses. It is also indirectly linked to cell membrane transporters like H+-ATPase and V-ATPase B.\n\nConclusionsThough carbonic anhydrase is not directly linked with EMS1 as revealed by network analyses and protein-protein interaction there could be a suitable condition generated by the carbonic anhydrase for EMS1 to be active. Hence, we report that carbonic anhydrase, along with other pH regulating gene complexes plays a major role for making EMS1 functional.

systems biology↗

bioRxiv2018

FabElm_BarcodeDb: matK barcode database of legumes

BackgroundDNA barcoding is an imperative implementation of chloroplast rbcL and matK regions exploited as standard molecular barcodes for species identification. MatK is highly conserved in plants and has been used extensively as a phylogenetic marker for classification of plants. In this study matK sequences of Leguminosae were retrieved for variant analysis and phylogentics. From online resources, maturase sequences were retrieved; redundant sequences and partials along with poor quality reads were filtered to compile 3639 complete non-redundant matK sequences and constructed into a database for ready reference. The database FabElm_BarcodeDb made available at app.bioelm.com was constructed using available sequence resources.\n\nResultsThe chloroplast genome of plants contains matK gene of 1500 bp, positioned between intron of trnK associated in-group II intron splicing. Mitochondrial matR and genomic matN sequences were compared with chloroplast matK. These maturase sequences share regions of homology with chloroplast and mitochondrial regions and are expected to be regulated by miRNA in producing splice variants contributing to speciation.\n\nConclusionBase substitution rates of nuclear maturase were comparable with mitochondrial maturase and are different from matK sequences. Hence, few identified species in this investigation were clustered with other tribes when analysed using matK. MatK is effective in resolving the species level variations as splicing contributes to speciation; but utilization of matK alone as a barcode marker for legumes is dubious, as it could not resolve some species identity.\n\nAbbreviations

plant biology↗