Biology subjects

Yadav, U.

Publications and source records attributed to Yadav, U..

3 recordsLinked to original sources

bioRxiv2019

Molecular Screening of Hemoglobin S Variant in Anemia Patients of Eastern UP Population

Hemoglobinopathies are the most common type of inherited disease in human. in India the most frequent and clinically significant hemoglobin structural variants are HbS, HbD and HbE. The HbS mutation, in which a glutamic acid at position 6 in the {beta} chain is substituted for valine Sickle cell disease is a major health problem in some parts of India. 2 ml blood sample was collected from 350 anemia patient and PCR-RFLP method was used for hemoglobin S analysis. Out of 350 samples, in four individuals, HbS mutation was found in homozygous ({beta} 6/{beta} 6) condition. All four individuals are Sickle cell cases. In conclusion, the percentage of Sickle cell disease was observed as 1.14% in Eastern UP anemic patients.

molecular biology↗

bioRxiv2019

A Study of Association of ABO Blood Group types with Cancer Risk

More than 30 blood group systems have been recognized by International Society of Blood Transfusion (ISBT). ABO blood group is one of the most studied blood group system. ABO blood group system consist of three alleles A, B and O, out which A and B are co-dominant and O is recessive. Many researchers and investigators have found association between ABO blood group and cancer risk. It was found from the recent data that blood group A and AB is associated with increased pancreatic and gastric cancer risk. In the present study data of ABO blood group of 243 patients, both males and females, with confirmed cases of cancer was obtained from Sir Sunderlal hospital, Institute of Medical Science (IMS), Banaras Hindu University (BHU) and Apex hospital, DLW Road, Varanasi. 250 Samples of both males and females were taken as control. Out of 243 cancer patients 117 were males and 126 were females. In 243 cases enrolled in present study, highest number of cases were of breast cancer among women and lowest were rectal cancer. It was found that A blood group was associated with breast cancer, oral cancer, liver cancer and ovarian cancer as compared to other blood group and blood group O was associated with lung cancer, gastric cancer, colon cancer, skin cancer and endometrial cancer.

genetics↗

bioRxiv2019

DRD2 TaqI A polymorphism in Eastern Uttar Pradesh population

Dopamine receptor D2 (DRD2) encoded by DRD2 gene, is located on chromosome 11q22-23. Dopamine plays the central role in motivation, cognition, and reward seeking behaviour. Its dysfunction is implicated in numerous neurological and psychiatric disorders including drug abuse, schizophrenia, ADHD etc. The TaqI A polymorphism is localized 9.8 kb downstream from DRD2 gene in exon 8 of protein kinase gene (ANKK1). It is a SNP demonstrated to cause Glutamate to Lysine substitution at 713 amino acid residue in putative binding domain of ANKK1. Due to the central role of dopamine in reward seeking behavior, DRD2 TaqI A loci is a suitable candidate for investigation of molecular basis of addiction. The aim of the present study is to evaluate the frequency of DRD2 TaqI A polymorphism in Eastern Uttar Pradesh population. 3ml blood samples were collected from 50 individuals randomly selected from Eastern UP. Written informed consent along with profile detail was taken from each subject prior to blood sample collection. DRD2 TaqI A polymorphism analysis was done by PCR-RFLP method. Genomic DNA was extracted from each collected blood samples and amplified using DRD2 Taq1 region specific primers. PCR amplification produced 310bp long amplicon which was digested with Taq I enzyme for polymorphism analysis. In case of A2 allele, Taq1 enzyme cleaved 310bp long fragment into two fragments of 180bp and 130bp. In case of A1 allele, a C to T substitution demolished the restriction site of Taq1, so amplicon of A1 allele remained uncut. In total 50 sample analyzed in present study, A2/A2, A2/A1 and A1/A1 genotype were found in 12, 32 and 06 samples respectively. The genotypic frequencies of mutant homozygous (A1/A1) is 0.12, heterozygous (A2/A1) is 0.64 and normal homozygous (A2/A2) is 0.24. The allelic frequency of A1 is 0.44 and of A2 is 0.56. In conclusion, the results of present study suggests that in TaqI A polymorphism of DRD2 gene, the frequency of allele A2 is higher than that of A1 allele in population of Eastern Uttar Pradesh.

neuroscience↗