Behavior of Neural Cells Post Manufacturing and After Prolonged Encapsulation within Conductive Graphene-Laden Alginate Microfibers
Engineering conductive 3D cell scaffoldings offer unique advantages towards the creation of physiologically relevant platforms with integrated real-time sensing capabilities. Toward this goal, rat dopaminergic neural cells were encapsulated into graphene-laden alginate microfibers using a microfluidic fiber fabrication approach, which is unmatched for creating continuous, highly tunable microfibers. Incorporating graphene increases the conductivity of the alginate microfibers 148%, creating a similar conductivity to native brain tissue. Graphene leads to an increase in the cross-sectional sizes and porosities of the fibers, while reducing the roughness of the fiber surface. The cell encapsulation procedure has an efficiency rate of 50%, and of those cells, approximately 30% remain for the entire 6-day observation period. To understand how encapsulation effects cell genetics, the genes IL-1{beta}, TH, TNF-, and TUBB-3 are analyzed, both after manufacturing and after encapsulation for six days. The manufacturing process and combination with alginate leads to an upregulation of TH, and the introduction of graphene further increases its levels; however, the inverse trend is true of TUBB-3. Long-term encapsulation shows continued upregulation of TH and of TNF-, and six-day exposure to graphene leads to the upregulation of TUBB-3 and IL-1{beta}, which indicates increased inflammation.