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Whicher, C.

Publications and source records attributed to Whicher, C..

2 recordsLinked to original sources
bioRxiv2018

Glycosylation-dependent modulation of the lL-2 signaling axis determines Th17 differentiation and IL-10 production

Metabolism plays an essential role in shaping T helper (Th) cell responses including the production of pro-inflammatory cytokines, however the effects on IL-10 have not been investigated. We show that the glucose analogue 2-deoxyglucose (2DG) specifically inhibits Th1 and Th2 cell differentiation and accompanying IL-10 production. In contrast, 2DG promotes IL-17A production by Th17 cells, even in the presence of IL-2 known to limit Th17 differentiation, whilst totally abrogating the production of IL-10. Notably, rather than inhibiting glycolysis, 2DG acts through the inhibition of glycosylation, which is critical for IL-2R surface expression and downstream signaling in both mouse and man. Strikingly, IL-2 is essential for IL-10 production by Th17 cells, in contrast to its inhibitory effect on the production of IL-17A. Our study reveals a previously unappreciated, anti-inflammatory role for IL-2 in Th17 cell production of IL-10 and thus identifies a novel mechanism to limit Th17 pathogenicity.

immunology↗
bioRxiv2016

10 Simple Rules for Sharing Human Genomic Data

Introduction Introduction Conclusion Competing Interests References Delivery of the promise of precision medicine relies heavily on human genomic data sharing. Sharing genome data generated through publicly funded projects maximises return on investment from taxpayer funds and increases the likelihood of obtaining funding in future rounds [1]. More importantly, genome data sharing makes it possible for other scientists to reuse existing datasets for further research and constitutes a direct measure of the current advancement in risk prediction, diagnosis, and treatment for genomic disorders [2].\n\nSharing of human genomic data carries responsibilities to protect confidentiality and the privacy of research participants [3]. In certain cases data sharing may be complicated or limited by agreements with ...

genomics↗