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Wenzlitschke, N.

Publications and source records attributed to Wenzlitschke, N..

2 recordsLinked to original sources

Absence of posterior commissure and sub-commissural organ precedes encephalocele development in a new mouse model

Encephalocele is a congenital defect involving herniation of the meninges, with or without brain tissue, outside the skull. Although traditionally considered a neural tube defect (NTD) alongside anencephaly and open spina bifida (myelomeningocele), encephalocele typically shows well-formed brain tissue, and is likely to represent a later-arising, post-neurulation developmental anomaly, with a different pathogenic mechanism from the open NTDs. A detailed understanding of encephalocele pathogenesis requires experimental studies and, recently, we developed a new mouse model in which the genes encoding FGF3, 4 and 15 are over-expressed in the embryonic day (E) 9.5 brain. Encephalocele subsequently develops as a broad forebrain-midbrain swelling, visible from E11.5, that resolves by birth into a focal brain herniation resembling human parieto-occipital encephalocele. A structural analysis of the brain in mutant embryos reveals absence of the posterior commissure and sub-commissural organ, and at later stages the pineal gland. These structures normally develop just rostral to the forebrain-midbrain boundary, and Pax6 immuno-histochemistry demonstrates that this boundary remains intact in the mutant embryos. Histological analysis reveals a more general change in tissue composition of the neural tube roof in the forebrain-midbrain region, with diminished thickness of the neuroepithelium and increased thickness of the overlying layer, including the non-neural ectoderm (future epidermis). We conclude that the posterior commissure and sub-commissural organ, previously implicated in hydrocephalus, may also be fundamental for development of the earlier-arising brain malformation, encephalocele.

developmental biology↗

Structural perturbation of chromatin domains with multiple developmental regulators can severely impact gene regulation and development

Chromatin domain boundaries delimited by CTCF motifs can restrict the range of enhancer action. However, disruption of domain structure often results in mild gene dysregulation and thus predicting the impact of boundary rearrangements on animal development remains challenging. Here, we tested whether structural perturbation of a chromatin domain with multiple developmental regulators can result in more acute gene dysregulation and severe developmental phenotypes. We targeted clusters of CTCF motifs in a domain of the mouse genome containing three FGF ligand genes--Fgf3, Fgf4, and Fgf15--that regulate several developmental processes. Deletion of the 23.9kb cluster that defines the centromeric boundary of this domain resulted in ectopic interactions of the FGF genes with enhancers located across the deleted boundary that are active in the developing brain. This caused strong induction of FGF expression and perinatal lethality with encephalocele and orofacial cleft phenotypes. Heterozygous boundary deletion was sufficient to cause these fully penetrant phenotypes, and strikingly, loss of a single CTCF motif within the cluster also recapitulated ectopic FGF expression and caused encephalocele. However, such phenotypic sensitivity to perturbation of domain structure did not extend to all CTCF clusters of this domain, nor to all developmental processes controlled by these three FGF genes--for example, the ability to undergo lineage specification in the blastocyst and pre-implantation development were not affected. By tracing the impact of different chromosomal rearrangements throughout mouse development, we start to uncover the determinants of phenotypic robustness and sensitivity to perturbation of chromatin boundaries. Our data show how small sequence variants at certain domain boundaries can have a surprisingly outsized effect and must be considered as potential sources of gene dysregulation during development and disease.

developmental biology↗