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Biology subjects

Weinstein, T.

Publications and source records attributed to Weinstein, T..

3 recordsLinked to original sources

Bioelectronic wearable sensors produced by computerized embroidery using a dual thread approach

Electronic textiles offer a promising route to wearable systems capable of continuous biophysical and biochemical monitoring. Their broader adoption remains limited by conductive threads that rarely support computerized embroidery (CEmb), reliable electronic integration, and reduced electrochemical capabilities due to coatings that degrade under bending, friction, or aqueous exposure. In this work, we use a dual-thread strategy that integrates silver-plated polyamide threads (AgPT) for high-conductivity pathways with PECOTEX, a chemically resilient PEDOT:PSS-coated cotton thread optimized for biochemical sensing. This approach preserves compatibility with CEmb, enhances electrochemical performance, and reduces cost by reserving AgPT for critical traces. We demonstrate a CEmb-compatible fabrication process for two wearable devices: a fully embroidered SmartBra for electrocardiography and a two-electrode ion sensor for potassium and sodium detection. We validate the design of our wearable dual-thread biosensors through laboratory characterization experiments and evaluate electrochemical sensing performance using a breast milk substitute, enabling realistic testing within womens health and infant-nutrition contexts. By focusing on scalable fabrication and meaningful applications, this work advances embroidered electronic textile systems toward practical, personalized healthcare monitoring.

bioengineering↗

Electrochemical Lateral Flow Assay with Linked Analytics for the Surveillance of Cassava Brown Streak Disease in East Africa

Cassava brown streak disease (CBSD) severely threatens food security in East Africa and the livelihoods of hundreds of millions globally. Effective control requires large-scale surveillance in resource-limited settings, which is currently lacking. We present ELLA (Electrochemical Lateral Flow Assay with Linked Analytics), a portable, battery-free, low-cost digital diagnostic platform integrating lateral flow assays with near-field communication and electrochemical readouts for cloud-based data storage and analytics. Validated through extensive field trials in East Africa and smaller studies in Brazil, ELLA achieved 89% agreement with RT-qPCR and 95% with ELISA, often surpassing ELISA sensitivity at a material cost below US$1 per test. Leveraging ELLAs molecular results, we trained a deep-learning model (DeepELLA) for rapid, image-based diagnosis of CBSD, enabling scalable surveillance of this and potentially emerging plant pathogens. By combining electrochemical sensing, digital connectivity, and AI-driven analytics, ELLA offers a powerful tool to strengthen plant disease monitoring and food security. Its modular design also allows adaptation to other chemical and biological targets, creating opportunities for novel datasets and new insights into plant and environmental health.

bioengineering↗

Pathogenic strains of a gut commensal drive systemic platelet activation and thromboinflammation in lupus nephritis

Imbalances in the gut microbiome have been linked to increased intestinal permeability and disease flares in systemic lupus erythematosus (SLE). Our study revealed that patients with flares of lupus nephritis (LN) and intestinal expansions of the anaerobic commensal, Ruminococcus gnavus (RG), displayed whole blood transcriptome profiles indicative of platelet, neutrophil, and myeloid cell activation, a profile reminiscent of sepsis. Serum analysis confirmed elevated serum levels of Platelet Factor 4 and neutrophil extracellular traps, which significantly correlated with levels of IgG-antibody to a novel lipoglycan (LG) produced by pathogenic RG strains, which was also documented in an independent LN cohort. To test for causality, in vivo mouse models further demonstrated that gut colonization with LG-producing RG strains, as well as a single intraperitoneal challenge with an LG preparation, caused platelet activation and megakaryocytosis in bone marrow and spleen. Mice colonized with RG strains that produce LG developed cellular infiltration of the kidneys by neutrophils and monocytes. Hence, RG expansions during renal flares may identify a specific LN flare endotype driven by thromboinflammatory mechanisms. Antibodies that arise from immune exposure to the RG lipoglycan may serve as a surrogate biomarker, helping to elucidate the impact of the relationship between gut microbiota communities and clinical outcomes in patients afflicted by LN. [208] Graphical Abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=164 SRC="FIGDIR/small/641288v1_ufig1.gif" ALT="Figure 1"> View larger version (74K): org.highwire.dtl.DTLVardef@1239f8eorg.highwire.dtl.DTLVardef@1c062caorg.highwire.dtl.DTLVardef@195ece2org.highwire.dtl.DTLVardef@1f3123f_HPS_FORMAT_FIGEXP M_FIG C_FIG

immunology↗