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Biology subjects

Vissers, J. P.

Publications and source records attributed to Vissers, J. P..

2 recordsLinked to original sources

Advances in the Design and Functionality of a Compact Multi-Reflecting Time-of-Flight Mass Spectrometer

AO_SCPLOWBSTRACTC_SCPLOWO_ST_ABSRationaleC_ST_ABSHigh-resolution mass spectrometry is routinely used for the analysis of complex samples in pharmaceutical, environmental, and omics related studies. Such applications demand instrumentation to be capable of combining sub-ppm mass accuracy, high resolving power, rapid full m/z range acquisition, over a wide dynamic range. MethodsAchieving the above requirements places constraints on analyzer design and performance. Multi-reflecting time-of-flight (MRT) based analyzers have previously been reported as a means of significantly extending the effective flight path in compact TOF designs. Here, further instrument and functionality advances in a compact MRT mass spectrometer design are described. ResultsThe impact of these enhancements was assessed for targeted and non-targeted omics applications, examining the impact of acquisition speed on resolving power, dynamic range including limits of quantitation, and quantitative precision. ConclusionThe results obtained characterize the performance of the enhanced design features of a compact MRT mass spectrometer. Operation at elevated acquisition rates up to 200 Hz was observed without loss in resolving power, isotopic ratio accuracy, or quantitative precision.

biochemistry↗

Development of a General Purpose Targeted LC-MS Method for Accurate Quantification of the SARS-CoV-2 Spike Protein Expression

The COVID-19 pandemic has catalyzed interest in immuno-multiple reaction monitoring (immuno-MRM) methods, with the detection of peptides unique to the nucleocapsid protein in nasopharyngeal swabs. While current applications predominantly focus on disease biomarkers, the pandemic has unveiled new opportunities, namely for the quantification of antigen expression following mRNA vaccination. Here, we present an optimized immuno-MRM method for quantifying SARS-CoV-2 spike protein fusion peptide, SFIEDLLFNK, for several practical applications. The method is versatile, applicable to multiple biological matrices, including plasma, and can be extended to nasopharyngeal swabs. It also offers a high-precision tool for assessing protein expression following plasmid and mRNA transfection. Moreover, in parallel to enabling accurate antigen quantification, the flow-through can be used to determine the proteome profile of the infected cells, providing insights into the intracellular immune response. This dual capability supports the rapid optimization of mRNA vaccines, thereby driving advancements in vaccine development strategies.

immunology↗