Biology subjects

Vinel, L.

Publications and source records attributed to Vinel, L..

2 recordsLinked to original sources

bioRxiv2026

Parathyroid Hormone Receptor 1 Signaling Encodes Multifaceted Metabolic Programs in AgRP Neurons

Organisms continuously adapt food intake and nutrient utilization to fluctuating energy demands, requiring the brain to accurately integrate signals coming from diverse metabolic organs and local signals into distinct physiological responses. How hypothalamic neurons discriminate these signals to independently regulate feeding behavior and peripheral metabolism remains poorly understood. In recent years, a coordinated regulation between bone physiology and the central control of energy metabolism has emerged, opening questions about potential common hormonal networks and signaling pathways between the bone and the brain. Here, we identify parathyroid hormone receptor 1 (PTH1R) signaling, a master regulator of calcium ion biochemistry and bone formation, in Agouti-related peptide (AgRP) neurons as an unexpected neuroendocrine mechanism for the control of feeding behaviourand nutrient partitioning. the two cognate endocrine and paracrine ligands, PTH and PTH-related peptide (PTHrP), selectively activat segregated intracellular kinases activation and lipophagic processes in AgRP neuron, ultimately producing convergent physiological outputs. Together, our findings identify the parathyroid hormonal system acts as a fine-tuning mechanism in specialized neuronal circuits allowing for hypothalamic adaptive responses to energy demands and systemic metabolic signals. Keywords: Parathyroid hormone-related peptide (PTHrP), hypothalamus, arcuate nucleus, agouti-related peptide neurons, energy metabolism, fasting, autophagy, free fatty acids

neuroscience↗

bioRxiv2023

A first morphological and electrophysiological characterization of FCs of the mouse cerebellum.

The "feathered" cells of Fananas (FCs) are cerebellar glia of unknown function. Initially described more than a century ago, they have been practically absent from the scientific literature. Here, we combined whole-cell patch-clamp recordings, dye loading and near UV-laser photolysis for a first characterization of FCs in terms of their morphology, electrophysiological properties and glutamate-evoked currents. FCs were identified in cerebellar slices by their small cell bodies located in the molecular layer and stubby processes. Despite a more compact shape compared to Bergmann glia (BGs) FCs had similar membrane resistances and basal currents, suggesting electrical coupling between neighboring glia. Dye filling and pharmacological experiments confirmed homo- and heterotypic gap junction coupling among FCs and BGs. Parallel-fiber stimulation evoked in FCs a slow inward current partially blocked by NBQX, along with superimposed fast (ms) transients. Repetitive stimulations resulted in a rapid desensitization of this AMPA-receptor mediated current, which recovered for stimulation intervals >0.5s. Laser photolysis of MNI-caged glutamate replicated fast inward currents with highest current densities in the distant process. We conclude that FCs respond with fast AMPA currents to local glutamate release, and that they integrate ambient glutamate to a slow inward current. Interestingly, FCs prevail throughout adulthood with markedly different densities among cerebellar lobes. Thus, FCs are not just displaced BGs as previously suggested, but they may have lobule specific functions, both locally and at the circuit level, yet to be uncovered.

neuroscience↗