Biology subjects

Vigre, H.

Publications and source records attributed to Vigre, H..

3 recordsLinked to original sources

bioRxiv2026

Single-Cell Metagenomics Links Plasmid-Derived Antimicrobial Resistance to Hosts in the Pig Gut Microbiome

Antimicrobial exposure can alter gut resistance reservoirs, but bulk metagenomics alone often cannot distinguish whether observed changes reflect expansion of bacterial hosts, altered abundance of plasmid-derived sequences, or redistribution of mobile elements across host backgrounds. Here, we combined longitudinal bulk short-read metagenomics with selected bulk long-read and single-cell shotgun metagenomic sequencing to analyse faecal samples from six Danish pigs over 11 weeks, including an unplanned tiamulin exposure affecting the three pigs housed on the right side of the stable. We constructed a catalogue of 885 plasmid-derived sequences collapsed into 195 bins. Twenty-eight bins and 212 contigs carried resistance annotations, including ribosomal-target markers relevant to pleuromutilin exposure. Single-cell evidence linked subsets of plasmid-derived bins and contigs to bacterial host taxa, enabling host-resolved inspection of resistance-associated plasmid-derived features in longitudinal bulk metagenomes. The microbiome-wide plasmid-derived-sequence prevalence screen identified two bins with post-event associations, whereas resistance-gene abundance and host-attributed plasmid-derived-sequence abundance screens identified no significant host-resolved associations. Because exposure was unplanned and confounded with pen side and disease signs, treatment-response results are exploratory. The main contribution is a single-cell-informed microbial ecology workflow for linking plasmid-derived resistance features to host backgrounds and longitudinal abundance patterns in complex gut

microbiology↗

bioRxiv2018

Proficiency of WHO Global Foodborne Infections Network External Quality Assurance System participants in the identification and susceptibility testing of thermo-tolerant Campylobacter spp. from 2003-2012.

Campylobacter spp. are food- and water borne pathogens. While rather accurate estimates for these pathogens are available in industrialized countries, a lack of diagnostic capacity in developing countries limits accurate assessments of prevalence in many regions. Proficiency in the identification and susceptibility testing of these organisms is critical for surveillance and control efforts. The aim of the study was to assess performance for identification and susceptibility testing of thermo-tolerant Campylobacter among laboratories participating in the World Health Organization (WHO) Global Foodborne Infections Network (GFN) External Quality Assurance System (EQAS) over a nine year period.\n\nParticipants (primarily national level laboratories) were encouraged to self-evaluate performance as part of continuous quality improvement.\n\nThe ability to correctly identify Campylobacter spp. varied by year and ranged from 61.9 % (2008) to 90.7 % (2012), and the ability to correctly perform antimicrobial susceptibility testing (AST) for Campylobacter spp. appeared to steadily increase from 91.4 % to 93.6 % in the test period (2009-2012).\n\nPoorest performance (60.0 % correct identification and 86.8 % correct AST results) was observed in African laboratories.\n\nOverall, approximately 10 % of laboratories reported either an incorrect identification or antibiogramme. As most participants were (supra)-national reference laboratories, these data raise significant concerns regarding capacity and proficiency at the local, clinical level. Addressing these diagnostic challenges is critical for both patient level management and broader surveillance and control efforts.

microbiology↗

bioRxiv2017

Abundance and diversity of the fecal resistome in slaughter pigs and broilers in nine European countries

EFFORT groupHaitske Graveland, Alieda van Essen, Bruno Gonzalez-Zorn, Gabriel Moyano, Pascal Sanders, Claire Chauvin, Julie David, Antonio Battisti, Andrea Caprioli, Jeroen Dewulf, Thomas Blaha, Katharina Wadepohl, Maximiliane Brandt, Dariusz Wasyl, Magdalena Skarzynska, Magdalena Zajac, Hristo Daskalov, Helmut W Saatkamp, Katharina D.C. Stark.\n\nAbstractAntimicrobial resistance (AMR) in bacteria and associated human morbidity and mortality is increasing. Use of antimicrobials in livestock selects for AMR that can subsequently be transferred to humans. This flow of AMR between reservoirs demands surveillance in livestock as well as in humans. As part of the EFFORT project (www.effort-against-amr.eu), we have quantified and characterized the acquired resistance gene pools (resistomes) of 181 pig and 178 poultry farms from nine European countries, generating more than 5,000 gigabases of DNA sequence, using shotgun metagenomics. We quantified acquired AMR using the ResFinder database and a database constructed for this study, consisting of AMR genes identified through screening environmental DNA. The pig and poultry resistomes were very different in abundance and composition. There was a significant country effect on the resistomes, more so in pigs than poultry. We found higher AMR loads in pigs, while poultry resistomes were more diverse. We detected several recently described, critical AMR genes, including mcr-1 and optrA, the abundance of which differed both between host species and countries. We found that the total acquired AMR level, was associated with the overall country-specific antimicrobial usage in livestock and that countries with comparable usage patterns had similar resistomes. Novel, functionally-determined AMR genes were, however, not associated with total drug use.

microbiology↗