Vasconcelos, T. N. C. | Search bioRxiv

bioRxiv2023

Contrasting patterns of foraging behavior in Neotropical stingless bees using pollen and honey metabarcoding

Stingless bees are major flower visitors in the tropics, but their foraging preferences and behavior are still poorly understood. Studying stingless bee interactions with angiosperms is methodologically challenging due to the high tropical plant diversity and inaccessibility of upper canopy flowers in forested habitats. Pollen DNA metabarcoding offers an opportunity of assessing floral visitation efficiently and was applied here to understand stingless bee floral resources spectra and foraging behavior. We analyzed pollen and honey of three distantly related species of stingless bees, with different body size and social behavior: Melipona rufiventris, Scaptotrigona postica and Tetragonisca angustula. Simultaneously, we evaluate the local floristic components through seventeen rapid botanical surveys conducted at different distances from the nests. We discovered a broad set of explored floral sources, with 46.3 plant species per bee species in honey samples and 53.67 in pollen samples. Plant families Myrtaceae, Asteraceae, Euphorbiaceae, Melastomataceae and Malpighiaceae dominated the records, indicating stingless bee preferences for abundant resources that flowers of these families provide in the region. Results also reinforce the preference of stingless bees for forest trees, even if only available at long distances. Our high-resolution results encourage future bee-plant studies using pollen and honey metabarcoding in hyper diverse tropical environments.

ecology↗

bioRxiv2020

Underlying microevolutionary processes parallel macroevolutionary patterns in ancient Neotropical Mountains

AimThe exceptional species-richness associated with mountains worldwide is linked to the fragmented topography of these areas, responsible for constantly isolating populations during periods of climatic fluctuations. Consequently, endemism and spatial turnover in mountains are very high and few species are widespread among entire mountain ranges, precluding population-level studies that help understanding how macroevolutionary patterns were shaped. Here, we used the bromeliad Vriesea oligantha, a species endemic to, but widespread in, one of the most species-rich ancient montane areas in the globe, the Espinhaco Range, to test how environmental changes over time may have acted on the evolutionary history of this taxon, contributing to understanding how montane macroevolutionary patterns were shaped. Through analyses of plastidial and nuclear DNA of V. oligantha, we dated its origin and intraspecific diversification, and estimated the genetic diversity, structure and migration rates among populations. Using climatic and geographic variables, we modeled suitable areas for the present and the past, estimating corridors between isolated populations. We also used demographic analyses to estimate ancient population dynamics of V. oligantha. Finally, we tested whether climatic variables or geographical distance explain the observed population structure. The origin and intraspecific diversification of V. oligantha are related to early climatic oscillations during the Plio-Pleistocene. This species has a high population structure due to its low pollen and seed dispersibility. The analysis of species distribution modeling estimated corridors between populations in the past, whereas the structure of V. oligantha results from both models of isolation by distance and isolation by environment. The phylogeographic patterns of Vriesea oligantha reflect previously recognized spatial and temporal macroevolutionary patterns in the Espinhaco Range, providing insights into how microevolutionary processes may have given rise to this astonishing mountain biodiversity.

evolutionary biology↗