Modular core network constructed from Escherichia coli transcriptome datasets using a hypergraph-based pan-network approach
We have developed a method to integrate transcriptomic coexpression networks across diverse experimental conditions within a single species. Our framework expands previous pannetwork approaches into a hypergraph based pannetwork. It first identifies coexpressed gene clusters within each individual dataset and extracts frequently co-expressed gene sets across multiple datasets using a frequent itemset mining algorithm. This process yields a hypergraph where each hyperedge is assigned a frequency (universality, U). We then extracted a subnetwork comprising high U hyperedges as the core network and formed modules within it. We applied our method to 106 Escherichia coli transcriptome datasets from the GEO database. The modularity of the core network peaked at a universality cutoff of 15, which was subsequently used to define it. Approximately 70% of the resulting core modules correspond to operons, and conversely, approximately 70% of all operons are covered by these core modules. We visualized the core network via an inter-modular network and analyzed core module dataset relationships using a modularity profile matrix. Based on these analyses, we successfully visualized the dynamic reorganization of the coexpression network in response to environmental changes in bacteria.