Biology subjects
Publications and source records attributed to Tveiten, H..
Withdrawal statementThe authors have withdrawn their manuscript owing to major modifications. Therefore, the authors do not wish this work to be cited as reference for the project. If you have any questions, please contact the corresponding author.
Cultivation of sterile-only fish in aquaculture offers multiple benefits of environmental, economical, and social value. A reliable method for efficient sterilization without affecting fish welfare and performance traits would have significant impact on fish production practices. Here, we demonstrate sterilization of Atlantic salmon embryos by targeting the dead end gene with antisense oligonucleotides. Successful gene knock down and sterilization was achieved only when using Gapmer oligonucleotides and not with morpholino oligos. Germ cell-depleted embryos developed into morphologically normal male and female salmon with rudimentary gonads devoid of gametes.