Biology subjects

Tunterak, W.

Publications and source records attributed to Tunterak, W..

2 recordsLinked to original sources

bioRxiv2025

Evidence of Human adaptation for cluster 2 TMUV strain despite effective anti-viral responses.

Tembusu virus (TMUV) is an emerging Orthoflavivirus with potential zoonotic importance. To date, the biological differences among TMUV strains remain poorly understood. In this study, we compared the replication of a TMUV strain belonging to the cluster 1 and one belonging to the cluster 2 in human lung epithelial cells and further examined the replication characteristics of TMUV cluster 2 strain in both human and mosquito cell lines. The TMUV cluster 2 strain demonstrated a significant higher replication efficiency than the cluster 1 strain in human cells, indicating stronger adaptation to human-derived cells. Infection of human cells with the cluster 2 strain also induced robust innate immune response, including type I interferon and interferon-stimulated gene expression, associated with activation of the unfolded protein response via the PERK, and IRE1 pathways, reflecting an ER stress engagement. These findings suggest that TMUV cluster 2 strain exhibits enhanced replication capacity and more effectively modulates human host responses at the cellular level, underscoring its potential relevance in human health and the zoonotic risk it represents.

molecular biology↗

bioRxiv2025

Poor sleep impairs immune responses and influenza vaccine protection

Disrrupted sleep, a common occurrence among shift workers, older adults, and individuals with sleep disorders, is increasingly recognized as a potential factor interfering with vaccine efficacy. Using a mouse model, we show that two weeks of chronic sleep fragmentation (CSF) before and during influenza vaccination significantly impair humoral immunity and reduce protection against lethal viral challenge. CSF-exposed mice exhibited lower neutralizing antibody titers, diminished IgG subclass responses, and reduced survival after viral challenge, despite preserved antibody binding avidity. Single-cell RNA sequencing and immune receptor profiling revealed altered B cell maturation, abnormal germinal center activation, and plasma cell stress, characterized by activation of unfolded protein response and oxidative stress pathways. CSF also reprogrammed B-cell signaling and disrupted coordination with T-cells. Together, this study showed that CSF compromises vaccine-induced immune responses by affecting multistage of B-cell differentiation, underscoring the importance of considering sleep health in vaccination strategies for vulnerable populations. One Sentence SummaryPoor sleep weakens both the magnitude and quality of immune responses, compromising the protective efficacy of influenza vaccination.

immunology↗