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Biology subjects

Tufenkji, N.

Publications and source records attributed to Tufenkji, N..

3 recordsLinked to original sources

Gender differences in submission behavior exacerbate publication disparities in elite journals

Women are particularly underrepresented as leading authors of papers in journals of the highest impact factor, with substantial consequences for their careers. While a large body of research has focused on the outcome and the process of peer review, fewer articles have explicitly focused on gendered submission behavior and the explanations for these differences. In our study of nearly five thousand active authors, we find that women are less likely to report having submitted papers to journals of the highest impact (e.g., Science, Nature, or PNAS) andto submit fewer manuscripts, on average, than men when they do submit. Women were more likely to indicate that they did not submit their papers (in general and their subsequently most cited papers) to high-impact journals because they were advised not to. In the aggregate, no statistically significant difference was observed between men and women in how they rated the quality of their work. Nevertheless, regardless of discipline, women were more likely than men to indicate that their "work was not ground-breaking or sufficiently novel" as a rationale for not submitting to one of the listed prestigious journals. Men were more likely than women to indicate that the "work would fit better in a more specialized journal." We discuss the implications of these findings and interventions that can serve to mitigate the disparities caused by gendered differences in submission behavior. SignificancePublishing in high-impact scholarly journals has a significant effect on researchers careers. Our findings identify factors that affect submission to Science, Nature, and the Proceedings of the National Academy of Sciences of the United States of America (PNAS) and explore whether there is a relationship between gender and desk rejections or submission rates. We found no relationship between gender and reported desk rejection and a relationship between gender and reported submissions, with men having a greater number of submissions. Women were more likely than men to indicate that their "work was not ground-breaking or sufficiently novel" for the listed prestigious journals and that they were advised against submitting to these venues. Men were more likely to indicate that the "work would fit better in a more specialized journal."

scientific communication and education↗

Single particle-resolution fluorescence microscopy of nanoplastics

Understanding of nanoplastic prevalence and toxicology is limited by imaging challenges resulting from their small size. Fluorescence microscopy is widely applied to track and identify microplastics in laboratory studies and environmental samples. However, conventional fluorescence microscopy, due to diffraction, lacks the resolution to precisely localize nanoplastics in tissues, distinguish them from free dye, or quantify them in environmental samples. To address these limitations, we developed techniques to label nanoplastics for imaging with Stimulated Emission Depletion (STED) microscopy to achieve resolution at an order of magnitude superior to conventional fluorescence microscopy. These techniques include (1) passive sorption; (2) swell incorporation; and (3) covalent coupling of STED-compatible fluorescence dyes to nanoplastics. We demonstrate that our labeling techniques, combined with STED microscopy, can be used to resolve nanoplastics of different shapes and compositions as small as 50 nm. The longevity of the dye labeling is demonstrated in different media and conditions of biological and environmental relevance. We also test STED imaging of nanoplastics in exposure experiments with the model worm C. elegans. These techniques will allow more precise localization and quantification of nanoplastics in complex matrices. SynopsisWe show that Stimulated Emission Depletion (STED) microscopy can be used to image single nanoplastics of different compositions and shapes. This will allow researchers to study environmentally-relevant nanoplastics and their interactions with organisms in relevant exposure scenarios. TOC Graphic O_FIG_DISPLAY_L [Figure 1] M_FIG_DISPLAY C_FIG_DISPLAY

ecology↗

Mechano-bactericidal nanopillars require external forces to effectively kill bacteria

Nanopillars are known to mechanically damage bacteria, suggesting a promising strategy for highly-effective anti-bacterial surfaces. However, the mechanisms underlying this phenomena remain unclear, which ultimately limits translational potential towards real-world applications. Using real-time and end-point analysis techniques, we demonstrate that in contrast to expectations, bacteria on multiple "mechano-bactericidal" surfaces remain viable, unless exposed to a moving air-liquid interface which caused considerable cell death. Reasoning that normal forces arising from surface tension may underlie mechano-bactericidal activity, we developed computational and experimental models to estimate, manipulate, and recreate the impact of these forces. Our experiments together demonstrate that nanopillar surfaces alone do not cause cell death, but require a critical level of external force to deform and rupture bacteria. These studies hence provide fundamental physical insight into the mechanisms by which nanopillar surfaces can serve as effective antibacterial strategies, and describe the use-conditions under which such nanotechnological approaches may provide practical value. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=107 SRC="FIGDIR/small/012153v1_ufig1.gif" ALT="Figure 1"> View larger version (29K): org.highwire.dtl.DTLVardef@1cfded2org.highwire.dtl.DTLVardef@2dc47dorg.highwire.dtl.DTLVardef@bf002dorg.highwire.dtl.DTLVardef@1a49c2d_HPS_FORMAT_FIGEXP M_FIG Graphical Abstract C_FIG

microbiology↗