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Biology subjects

Trivino, M.

Publications and source records attributed to Trivino, M..

2 recordsLinked to original sources

Ecologically and economically sustainable level of timber harvesting in boreal forests - defining the safe operating space for forest use

Planetary-level analyses indicate that we are exceeding the ecological limits. However, we need approaches to implement global sustainability frameworks regionally, using natural resources at levels that allow for their regeneration. We present a framework to define limits beyond which ecosystems are threatened to collapse, to answer how much we can extract from ecosystems, and to manage natural resources for both human and ecosystem wellbeing. We exemplify this approach with the heath forest habitat types in Finland, which provide most of the national timber production. We use the IUCN Red List of Habitats to set favourable reference values for volume of deadwood, proportion of old-growth forest cover and proportion of deciduous trees. Through forest growth simulations and management optimization, we found that the proportion of old-growth forest is the most challenging criteria to be reached only by 2100. This would require not only a larger use of extensive forest management practices than hitherto but also to drastically reduce the maximum economic sustainable harvest level from the current 96% to 60%. By combining threat assessments with ecosystem modelling and management planning, this approach can support regional decision makers to make informed decisions to stay within safe limits of use of natural resources.

ecology↗

GPI anchor remodeling by the plant PGAP1 ortholog HLD1 is essential for Papaver self-incompatibility

In eukaryotes, glycosylphosphatidylinositol anchored proteins (GPI-APs) are tethered to the outer leaflet of the plasma membrane where they function as key regulators of a plethora of biological processes. Self-incompatibility (SI) plays a pivotal role regulating fertilization in higher plants through recognition and rejection of self pollen. Here we used Arabidopsis thaliana lines engineered to be self-incompatible by expression of Papaver SI determinants for an SI suppressor screen. We identify HLD1, an ortholog of human GPI-inositol deacylase PGAP1, whose mutation completely abolishes the SI response. We show that HLD1 functions as a GPI-inositol deacylase and that this GPI-remodeling activity is essential for SI. Using GFP-SKU5 as a representative GPI-AP, we show that HLD1 mutation does not affect GPI-AP production and targeting, but alters the configuration of mature GPI-APs. This prevents GPI-AP release from the plasma membrane, suggesting that this process plays a critical role in the regulation of SI. Our data not only identify GPI anchoring as a new pathway of SI providing new directions to investigate SI mechanisms, but identifies for the first time a function for GPI-AP remodeling by inositol deacylation in plants. One sentence summaryThe Papaver self-incompatibility response requires GPI-anchor modification by HLD1, an ortholog of the mammalian PGAP1.

plant biology↗