Biology subjects

Traore, A.

Publications and source records attributed to Traore, A..

3 recordsLinked to original sources

bioRxiv2025

Genomic diversity, population structure and admixture in native cattle breeds of Benin

This study investigates the genetic diversity, population structure, and admixture patterns of indigenous cattle breeds in Benin, shedding light on their evolutionary relationships and adaptation to the West African environment. A total of 348 cattle from eight indigenous breeds of Benin, including taurine (Lagune, Borgou, Pabli, and Somba), zebu (Gudali, Zebu Peuhl, and Yakana) and one crossbred (Bourgou X Zebu) cattle were genotyped along with a reference dataset of cattle from Europe, Asia, and West Africa. After quality control, 28.591 SNPs from 838 cattle were analyzed for genetic diversity, differentiation, and admixture. Pairwise FST values revealed significant genetic differentiation between local taurine and zebu breeds (FST = 0.05 - 0.15), with some populations showing close genetic relationships, while others, such as the Borgou and NDama breeds, exhibiting relatively more divergence. The admixture analysis indicated significant gene flow from zebu cattle into local taurine breeds, suggesting adaptive introgression driven by factors such as heat tolerance and disease resistance. Additionally, the effective population size (Ne) was relatively higher in Benins taurine breeds as compared to zebu, likely attributable to traditional open communal mating practices. The genetic structure also reflected the influence of both historical and ongoing introgression from Asian zebu cattle. The results highlight the importance of maintaining genetic diversity through regional breeding strategies that consider environmental and adaptive pressures. The results of the present study will serve as a basis for the development of Community Based Breeding Programs (CBBPs) for Beninese cattle adapted to local contexts, integrating information on admixture levels, breeders preferences, production performance, and the conservation of local genetic diversity.

genomics↗

bioRxiv2025

Long-term patient-derived ovarian cancer organoids closely recapitulate tumor of origin and clinical response

There is an urgent need of precision medicine for ovarian cancer patients to identify patients who respond to chemotherapy and PARP inhibitors, a therapy targeting homologous recombination deficiency (HRD). Here we established a panel of 37 long-term patient-derived tumor organoids (PDTO) models of various histological subtypes from 224 patients and demonstrated that they mimic the histological and molecular characteristics of original tumors. Screening of chemotherapeutic drugs showed that PDTO exhibit heterogeneous responses, and that response of PDTO from high-grade serous ovarian carcinoma to carboplatin recapitulated patient response to first-line treatment. Additionally, the detection of HRD phenotype of PDTO using functional assay was associated with the results of the HRD test Genomic Instability Scar (GIScar). Although larger-scale investigations are needed to confirm the predictive potential of PDTO, these results provide further evidence of the potential interest of ovarian PDTO for functional precision medicine even if many challenges remain to be addressed.

cancer biology↗

bioRxiv2021

Efficient Fluorescence-Based Localization Technique for Tracking Endophytes Route in Host-Plants Colonization

Bacterial isolates that enhance plant growth and suppress plant pathogens growth are essential tools for reducing pesticide applications in plant production systems. The objectives of this study were to develop a reliable fluorescence-based technique for labeling bacterial isolates selected as biological control agents (BCAs) to allow their direct tracking in the host-plant interactions, understand the BCA localization within their host plants, and the route of plant colonization. Objectives were achieved by developing competent BCAs transformed with two plasmids, pBSU101 and pANIC-10A, containing reporter genes eGFP and pporRFP, respectively. Our results revealed that the plasmid-mediated transformation efficiencies of antibiotic-resistant competent BCAs identified as PSL, IMC8, and PS were up 84%. Fluorescent BCA-tagged reporter genes were associated with roots and hypocotyls but not with leaves or stems and were confirmed by fluoresence microscopy and PCR analyses in colonized Arabidopsis and sorghum. This fluorescence-based techniques high resolution and reproducibility make it a platform-independent system that allows tracking of BCAs spatially within plant tissues, enabling assessment of the movement and niches of BCAs within colonized plants. Steps for producing and transforming competent fluorescent BCAs, as well as the inoculation of plants with transformed BCAs, localization, and confirmation of fluorescent BCAs through fluorescence imaging and PCR, are provided in this manuscript. This study features host-plant interactions and subsequently biological and physiological mechanisms implicated in these interactions. The maximum time to complete all the steps of this protocol is approximately three months. SENTENCE SUMMARYWe describe a novel fluorescence localization technique as a powerful tool to directly visualize and determine the route in-situ of BCAs in host-plants interaction. The study features the host-plant interactions, biological and physiological responses implicated.

cell biology↗