Biology subjects

Tominaga, J.

Publications and source records attributed to Tominaga, J..

3 recordsLinked to original sources

bioRxiv2026

Autopsy-based longitudinal multi-organ high-dimensional profiling reveals lineage plasticity in TRK-inhibitor-resistant secretory breast carcinoma

Secretory breast carcinoma (SBC) is typically indolent, yet mechanisms underlying aggressiveness and therapeutic resistance to tropomyosin receptor kinase inhibitors (TRKi) remain unclear. Autopsy-based longitudinal multi-organ high-dimensional profiling of metastatic TRKi-resistant SBC demonstrated histopathological heterogeneity, including secretory and squamous components, arising from a shared clonal origin. Integrated genomic and transcriptomic analyses revealed hierarchical transcriptional rewiring consistent with a lineage-plastic state, suggesting a potential link to tumor aggressiveness and therapeutic resistance.

pathology↗

bioRxiv2020

Robust estimates of cuticle conductance on stomatous leaf surfaces during the light induction of photosynthesis

O_LICuticle conductance (gcw) can bias calculations of intercellular CO2 concentration inside the leaf (Ci) when stomatal conductance (gsw) is small. C_LIO_LIWe examined how the light induction of photosynthesis impacts calculations by directly measuring Ci along with standard gas exchange in sunflower and tobacco leaves. C_LIO_LIWhen photosynthesis was induced from dark to saturating light (1200 mol m-2 s-1 PAR) the calculated Ci was significantly larger than measured Ci and the difference decreased as gsw increased. This difference could lead to over-estimation of rubisco deactivation by limited CO2 supply during early induction of photosynthesis. However, only small differences in Ci were observed during the induction from shade (50 mol m-2 s-1 PAR) because gsw was sufficiently large. The induction from dark also allowed robust estimations of gcw when combined with direct Ci measurements. These gcw estimates succeeded in correcting the calculation, suggesting that the cuticle was the major source of error. C_LIO_LIDespite a technical restriction to amphi-stomatous leaves, the presented technique has a potential to provide insights into the cuticle conductance on intact stomatous leaf surfaces. C_LI

plant biology↗

bioRxiv2020

Minimum conductance in leaves-cuticle, leaky stomata, or water vapor saturation?

O_LIMinimum conductance (gw,min) in leaves is important for water relations in land plants. Yet, its regulation is unclear due to measurement constraints. C_LIO_LICuticle conductance to water vapor (gcw) was estimated from the difference between calculated and direct measurement of CO2 concentration in the leaf airspace (Ci) of amphi-stomatous tobacco and sunflower. We estimated gcw in a series of light and dark experiments, and partitioned gw,min into cuticle and stomatal components. Some leaves were detached to simulate severe drought through desiccation conditions where gw,min is generally determined. C_LIO_LIBetween light and dark experiments each gcw was in close agreement, and successfully corrected the discrepancies of calculations from direct measurements. In the dark, either stomatal or cuticle conductance dominated the gw,min, suggesting either of them can control the minimum water loss. In the detached leaves, gcw could not be estimated likely due to unsaturation in the leaf airspace, and gw,min was progressively underestimated. C_LIO_LIBesides cuticle, leaf water status is a potential pitfall of the standard gas exchange model. Our technique is useful to study the minimal gas exchange as well as to refine the model. C_LI

plant biology↗