Tomasini, R. | Search bioRxiv

bioRxiv2020

DECONbench: a benchmarking platform dedicated to deconvolution methods for tumor heterogeneity quantification

MotivationQuantification of tumor heterogeneity is essential to better understand cancer progressionand to adapt therapeutic treatments to patient specificities. ResultsWe present DECONbench, a web-based application to benchmark computational methods dedicated to quantify of cell-type heterogeneity in cancer. DECONbench includes benchmark datasets, computational methods and performance evaluation. It allows submission of new methods. Availability and implementationDECONbench is hosted on the open source codalab competition platform. It is freely available at: https://competitions.codalab.org/competitions/23660. Supplementary informationAdditional information is available online and on our website: https://cancer-heterogeneity.github.io/deconbench.html.

bioinformatics↗

bioRxiv2020

Sympathetic axonal sprouting induces changes in macrophage populations and protects against pancreatic cancer

Recent evidence has highlighted the presence of neuronal nerve processes in the tumor microenvironment. However, the origin of intra-tumoral nerves remains poorly known, in part because of technical difficulties in tracing nerve fibers via regular histological preparations. Here, we employed three-dimensional (3D) imaging of cleared tissues for a comprehensive analysis of sympathetic innervation in pancreatic ductal adenocarcinoma (PDAC). The results support two independent, but coexisting, mechanisms: passive engulfment of pre-existing nerves within tumors and active, localized sprouting of nerve terminals into non-neoplastic lesions and tumor periphery. Nerve ablation revealed an inverse correlation between sympathetic innervation and tumor growth and spread. Furthermore, sympathectomy increased CD163+ macrophage levels, which contributed to worse outcomes. Altogether, our findings revealed protective properties of the sympathetic nervous system in PDAC immunity and progression that could pave the way for new treatments.

cancer biology↗