Biology subjects

Teder, T.

Publications and source records attributed to Teder, T..

2 recordsLinked to original sources

bioRxiv2025

Structural basis for ATP regulation of human 5-lipoxygenase

5-Lipoxygenase catalyzes the committed step in the biosynthesis of the powerful proinflammatory leukotrienes and pro-resolving anti-inflammatory lipoxins. Here we present a 3.3 [A] cryo-EM structure of wild type 5-lipoxygenase in complex with ATP, one of its most important allosteric regulators. The nucleotide is located in a positively charged pocket of the catalytic domain and held in place by a complex network of amino acid side chains and backbone carbonyl and amino groups. Mutagenetic analysis suggests that ATP binding and action is primarily mediated via Lys320, assisted by Gln657. Further atomistic simulations demonstrate that ATP binding induces movements of the PLAT domain coupled to conformational rearrangements at the active center. These findings provide important structural and regulatory insights to a key enzyme in leukotriene biosynthesis, thus aiding in design of new antiphlogistic drugs.

biochemistry↗

bioRxiv2021

Biodegradation of naphthalene mediated by the plant growth promoting rhizobacteria

We compared the ability of two bacterial strains, Paenibacillus polymyxa A26 and P. polymyxa A26Sfp, for biodegradation of naphthalene (NAP). The studies were performed under simulated laboratory conditions, in liquid medium and soil with different carbon sources, pH and salt contents. Changes in the luminescence inhibition of Aliivibrio fischeri, as an indicator of the baseline toxicity, were observed in degradation mixtures during 7 days of incubation. While both strains expressed the best growth and NAP degradation ability in the minimal salt medium containing sucrose and 5% NaCl at pH 8, the mutant strain remained effective even under extreme conditions. A26Sfp was found to be an efficient and potentially industrially important polycyclic aromatic hydrocarbon degradation strain. Its extracellular polysaccharide production is 30% and glucan production twice that of the wild type A 26. The surface tension reduction ability was ascertained as 25-30% increased emulsification ability.

microbiology↗