Biology subjects

Talbot, S. C.

Publications and source records attributed to Talbot, S. C..

3 recordsLinked to original sources

bioRxiv2024

Unlocking the Genetic Potential of Solanum bulbocastanum (SB22, Selection 22): A Valuable Resource for Enhancing Disease Resistance in Commercial Potato Cultivars

Cultivated potatoes are susceptible to a host of diseases caused by various pathogens. Wild relatives of potatoes are used in breeding programs as sources of resistance introgressed into cultivated potatoes. The wild potato Solanum bulbocastanum is an essential source of resistance to Columbia root knot nematode (CRKN) and late blight. We present the initial chromosome-level assembly of SB22, produced using PacBio long reads and Dovetail Hi-C scaffolding. The final assembly size was 655.3 Mb. Using the BRAKER pipeline, 43,280 gene models were predicted, with a BUSCO completeness of about 90.3%. Repeat elements represented 63.8% of the genome, with LTR elements being the most abundant. DRAGO3 predicted 2,310 disease resistance-like genes across the 12 chromosomes of SB22; the MEME suite was used to identify their amino acid motifs. Putative candidate genes contributing to CRKN resistance were mapped on chromosome 11 of SB22. The SB22 draft genome is a valuable genomic resource for potato breeding programs.

genomics↗

bioRxiv2024

A first look at the genome structure of hexaploid 'Black Mitcham' peppermint (Mentha piperita L.)

Peppermint, Mentha xpiperita L., is a hexaploid (2n = 6x = 72) and the predominant cultivar of commercial mint oil production in the US. This cultivar is threatened because of high susceptibility to the fungal disease Verticillium wilt, caused by Verticillium dahliae. This report details the first draft polyploid chromosome-level genome assembly for this mint species. The Black Mitcham genome resource will broaden comparative studies of disease resistance, essential oil biosynthesis, and hybridization events within the genus Mentha. It will also be a valuable contribution to the body of phylogenetic studies involving Mentha and other genera that contain species with varying ploidy levels.

genomics↗

bioRxiv2023

A haplotype-resolved chromosome-level assembly and annotation of European hazelnut (C. avellana cv. Jefferson) provides insight into mechanisms of eastern filbert blight resistance

European hazelnut (Corylus avellana L.) is an important tree nut crop. Hazelnut production in North America is currently limited in scalability due to Anisogramma anomala, a fungal pathogen that causes Eastern Filbert Blight (EFB) disease in hazelnut. Successful deployment of EFB resistant cultivars has been limited to the state of Oregon, where the breeding program at Oregon State University (OSU) has released cultivars with a dominant allele at a single resistance locus identified by classical breeding, linkage mapping, and molecular markers. Jefferson is resistant to the predominant EFB biotype in Oregon and has been selected by the OSU breeding program as a model for hazelnut genetic and genomic research. Here, we present a near complete, haplotype-resolved chromosome-level hazelnut genome assembly for C. avellana Jefferson. This new assembly is a significant improvement over a previously published genome draft. Analysis of genomic regions linked to EFB resistance and self-incompatibility confirmed haplotype splitting and identified new gene candidates that are essential for downstream molecular marker development, thereby facilitating breeding efforts.

genomics↗