Biology subjects

Tabatabaei Rezaei, N.

Publications and source records attributed to Tabatabaei Rezaei, N..

4 recordsLinked to original sources

bioRxiv2025

Bioprinting Taurine-Incorporated Gelatin Methacrylate Hydrogels for Enhanced Muscle Tissue Regeneration

Skeletal muscle diseases like myopathies and muscular dystrophies present significant clinical challenges with few effective treatments. To better understand disease mechanisms and accelerate therapy development, robust in vitro muscle models are needed. Extrusion- and light-based bioprinting offer precise fabrication of tissue-like constructs, but creating bioinks that support muscle cell function remains difficult. Here, we report a novel bioink in which taurine is first methacrylated--substituting its -NH2 groups to afford taurine methacrylate (TMA)--enabling covalent integration into gelatin methacryloyl (GelMA) networks. We systematically compared GelMA-Taur (physical blend) versus GelMA-TMA (covalent) hydrogels, assessing mechanical stiffness, swelling behavior, and photocrosslinking kinetics. Incorporating TMA yielded tighter crosslinking control, minimized overcure in DLP-printed features, and improved shape fidelity. SEM revealed finer pore structures and homogeneous TMA distribution, and release assays confirmed prolonged TMA retention compared to rapidly leaching taurine. Photopatterning and 3D printing of complex geometries demonstrated excellent printability of the GelMA-TMA bioink. Finally, C2C12 myoblasts encapsulated in GelMA-TMA scaffolds exhibited accelerated early differentiation, higher myosin heavy chain expression, and more extensive myotube formation than controls. Together, these results establish GelMA-TMA as a printable, mechanically tunable, and biologically active platform for engineering skeletal muscle tissue in disease modeling and regenerative applications.

bioengineering↗

bioRxiv2025

3D Bioprinting of Kidney Tissue Using a Photocrosslinkable Hydrogel Derived from Decellularized Extracellular Matrix

Three-dimensional bioprinting has emerged as a promising strategy in tissue engineering, aiming to fabricate functional tissue constructs for organ regeneration. A critical challenge in this field is the development of organ-specific bioinks that can provide a microenvironment conducive to cellular growth and differentiation. In this study, we successfully developed a photocrosslinkable bioink by methacrylating decellularized porcine kidney extracellular matrix. The decellularization process effectively removed all cellular components while preserving the native kidney extracellular matrix composition. The resulting methacrylated decellularized extracellular matrix bioink exhibited optimal rheological properties, making it well-suited for digital light processing based stereolithography and piston-driven extrusion bioprinting. Human embryonic kidney cells encapsulated in the bioink showed high viability and a strong proliferative capacity, indicating potential for tissue-specific maturation over time. This work demonstrates the feasibility of utilizing kidney-specific decellularized extracellular matrix-based bioinks, providing a platform for engineering renal tissue constructs for therapeutic applications.

bioengineering↗

bioRxiv2024

3D bioprinting of Liver Microenvironment Model Using Photocrosslinkable Decellularized Extracellular Matrix based Hydrogel

The liver, as one of the vital organs in the body, plays a crucial role in various bodily functions. Numerous factors can cause liver damage, that the sole remedy for severe liver conditions is the transplantation of healthy liver tissue. In response to the transplantation challenges, innovative approaches involving hydrogel-based technologies have emerged, leading to the creation of highly functionalized tissues. The development of three-dimensional printing and patterning of cell-laden biomaterial matrices offers promising advances for creating tissue-specific structures in tissue engineering and bioprinting. However, the matrix materials currently employed in bioprinting liver microtissue often fail to capture the complexity of the natural extracellular matrix (ECM), hindering their ability to restore innate cellular shapes and functions. Liver ECM-based hydrogels are increasingly recognized for their potential as biomimetic 3D cell culture systems that facilitate the exploration of liver disease, metabolism, and toxicity mechanisms. Yet, the conventional production of these hydrogels relies on slow thermal gelation processes, which restrict the manipulation of their mechanical characteristics. In this research, we introduce a novel approach with a functionalized photocrosslinkable liver decellularized extracellular matrix (dECM). By combining liver dECM methacrylate (LdECMMA) with gelatin methacrylate (GelMA), we achieved accelerated crosslinking under visible light irradiation and the ability to tune the mechanical, rheological, and physiological properties of the material. We encapsulated human hepatocellular carcinoma cells within an optimal concentration of the GelMA-LdECMMA hybrid hydrogel and examined cell proliferation and function over an extended period. The findings revealed that the GelMA-LdECMMA hybrid hydrogel enhances liver cell proliferation and function, holding significant promise for applications in drug screening and liver cancer metastasis research.

bioengineering↗

bioRxiv2023

Vascularized Liver Tissue Embedded Bioprinting Utilizing GelMA/Nanoclay-based Composite hydrogels

As the aging population grows, the need to regenerate non-self-repairing tissues becomes increasingly crucial for enhancing our quality of life. Tissue engineering offers a promising solution, particularly in recreating the intricate networks of blood vessels crucial for tissue vitality. These tissues rely on effective nutrient and oxygen circulation, with an optimal oxygen diffusion range of 100-200 {micro}m. Yet, crafting vascularized in vitro tissues remains a significant challenge. This study addresses the challenge by using GelMA-based hydrogels as a photocrosslinkable support bath, a biocompatible and versatile choice for biological applications. To enhance the rheological properties for in vitro tissue engineering, Laponite (LPN) is introduced as a rheology modifier. The study optimizes the GelMA-LPN nanocomposite hydrogel composition, ensuring the desired physical, mechanical, and rheological properties, including recovery. The research also explores the biological implications, encapsulating liver cells within the nanocomposite hydrogel, and studying their behavior under perfusion conditions. This research presents a promising avenue for creating vascularized in vitro tissues, potentially advancing tissue engineering and regenerative medicine.

bioengineering↗