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Szybinska, A.

Publications and source records attributed to Szybinska, A..

2 recordsLinked to original sources

Rpl40/eL40 ribosomal protein paralogs couple cytosolic translation to mitochondrial proteome and lipid homeostasis

Ribosomal protein paralogs are increasingly implicated in the regulation of cellular metabolism and mitochondrial function. However, the mechanisms linking paralog composition of ribosomes to mitochondrial physiology remain largely unclear. Here, we investigate the two Rpl40 paralogs in the budding yeast Saccharomyces cerevisiae and find that deletion of either paralog induces compensatory upregulation of the remaining gene and causes mild mitochondrial stress. Despite this shared phenotype, the mutants display distinct mitochondrial adaptations. Loss of Rpl40a is accompanied by increased abundance of mitochondrial proteins, including MICOS components, whereas loss of Rpl40b leads to reduced levels of mitochondrial inner membrane proteins, including the translocase Tim22 and carrier proteins, together with increased sensitivity to membrane stress. Notably, the two mutants show opposing changes in triglyceride abundance, pointing to paralog-specific control of lipid metabolic remodeling during mitochondrial stress. These findings suggest that Rpl40 paralogs differentially modulate cellular adaptation to mitochondrial stress, linking ribosome composition to mitochondrial proteostasis and lipid homeostasis.

biochemistry↗

p70S6 kinase-dependent phosphorylation of the μ2 subunit of the AP2 adaptor complex is needed for clathrin-mediated endocytosis.

Clathrin-mediated endocytosis (CME) internalizes cell-surface receptors via clathrin-coated invaginations of the plasma membrane. Both clathrin and endocytic cargo are recruited to these sites by the adaptor protein complex AP2. AP2 cycles between a closed cytoplasmic conformation and an open membrane-bound state, and efficient CME requires both conformations and their dynamic interconversion. The mechanisms regulating these conformational changes, which include post-translational modifications of the AP2, remain incompletely understood. Here, we report that p70S6 kinase phosphorylates the {micro}2 subunit of the AP2 and that the phosphorylation of serine 45 (S45) depends on p70S6K activity. Loss of S45-{micro}2 phosphorylation results in decreased internalization of canonical CME cargo such as transferrin and PDGF receptors. In Caenorhabditis elegans, lack of S45-{micro}2 phosphorylation produces directionally similar but markedly weaker phenotypes than AP2 loss of function. Live imaging and in silico dynamic modelling suggest that S45-2 phosphorylation has impact on the conformational changes of the AP2 complex. These findings identify a p70S6K-dependent mechanism that modulates AP2 function and further strengthen the importance of post-translational regulation in controlling CME. Summary statementClathrin-mediated endocytosis is essential for receptor internalization and cell signaling. This study identifies a novel regulatory mechanism in which p70S6K-mediated phosphorylation of the AP2 subunit 2 at Ser45 modulates AP2 conformational dynamics and facilitates receptor endocytosis.

cell biology↗