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Biology subjects

Swaminathan, P.

Publications and source records attributed to Swaminathan, P..

2 recordsLinked to original sources

MetaKnogic-Alpha: A Hyper-Relational Knowledge Base for Grounded Metabolic Reasoning

The exponential trajectory of biomedical literature has precipitated a fundamental "synthesis gap" in metabolic research, where critical mechanistic insights remain fragmented across hundreds of thousands of disjointed full-text articles, preventing the consolidation of a global mechanistic view. Here, we present MetaKnogic-Alpha, a foundational mechanistic knowledge substrate designed to bridge this gap by transforming unstructured literature into a navigable, logic-based resource. MetaKnogic-Alpha synthesizes over 100K full-text articles into a hyper-relational hypergraph structure, preserving the n-ary relational logic inherent in complex metabolic pathways. To ensure biological rigor, we implemented a hierarchical discovery protocol: an autonomous reasoning agent first enriches query nomenclature for domain-specific precision, followed by a multi-hop topological expansion within the hypergraph to surface functional neighbors, such as enzymatic co-factors and distal regulators, often lost in traditional search paradigms. Crucially, the system subjects all literature-derived insights to a deterministic biochemical grounding against a curated metabolic reaction network, significantly mitigating the risk of probabilistic hallucinations common in standalone generative models. In rigorous benchmarking, MetaKnogic-Alpha achieved a mechanistic accuracy of 0.98 in scenarios where supporting evidence was present, providing a robustly attributable audit trail back to the primary literature via PubMed Central Identifiers. We designate this primary release as "alpha" to establish the foundational architectural logic for a burgeoning million-scale resource. By compressing the synthesis of thousands of papers from a multi-month manual effort into several hours of automated discovery, MetaKnogic-Alpha serves as a high-fidelity research companion that augments the human experts ability to resolve complex metabolic interactions and identify novel therapeutic drivers in precision oncology.

bioinformatics↗

Analysis of patient data reveals novel cancer-relevant functions for GCN2/eIF2αK4

Numerous studies have shown that high GCN2 levels correlate with poor survival in a number of cancers. GCN2 has been known for over thirty years as a stress-response kinase, which phosphorylates the translation-initiation factor eIF2, and thereby contributes to reprogramming of translation. Here we performed correlation analyses of GCN2 expression and that of other genes in a patient-derived sample set of cervical cancer samples. We found correlations not only with genes involved in stress responses, but also with genes involved in mitosis and cell migration. Our functional analyses confirmed that these correlations indeed reveal novel functions. Furthermore, our analyses of growth benefits associated with elevated GCN2 levels suggest that the novel functions can contribute to aggressive disease in cancers with high GCN2 levels.

cell biology↗