Biology subjects

Suwanchaikasem, P.

Publications and source records attributed to Suwanchaikasem, P..

5 recordsLinked to original sources

bioRxiv2026

Pre-symptomatic proteomic and metabolomic profiling identifies compensated ER-redox-metabolic adaptation and early nuclear vulnerability in neuronal ERO1L toxicity

Aging progressively challenges neuronal proteostasis, redox homeostasis, and metabolism, yet the molecular changes that precede functional decline remain poorly understood. Endoplasmic reticulum oxidoreductin 1 (ERO1), a key regulator of oxidative protein folding, links endoplasmic reticulum (ER) proteostasis with cellular redox balance and is elevated in aging and neurodegenerative contexts. Here, we investigated how neuronal ERO1L elevation reshapes cellular homeostasis before overt dysfunction in Drosophila melanogaster. Endogenous ERO1L expression increased with age, and neuronal ERO1L elevation shortened lifespan and caused progressive locomotor decline. This effect was strongly cell-type dependent, as ERO1L elevation in glia, muscle, or fat body did not produce a comparable survival phenotype. At day 5 post-eclosion, locomotor performance remained preserved and major brain reactive-oxygen-species (ROS) accumulation was not yet detectable, defining a pre-symptomatic stage. Multi-omic profiling at this stage revealed selective remodelling of ER proteostasis, redox defence, and mitochondrial-energy pathways, together with changes in central-carbon, nitrogen, and purine metabolism. In contrast to these broadly adaptive responses, chromatin- and RNA-homeostasis-associated proteins were selectively reduced, accompanied by decreased HP1, dFmr1, and Piwi expression and increased transposable-element transcripts. Thus, neuronal ERO1L elevation establishes a pre-symptomatic state in which proteostatic and metabolic adaptation coexists with early vulnerability of nuclear and RNA-homeostasis pathways, preceding overt oxidative stress and behavioural decline. These findings provide an in vivo framework to investigate how age-associated ERO1L elevation may progressively reduce neuronal resilience during brain aging.

neuroscience↗

bioRxiv2026

Isolation and characterization of Bacopa monnieri (L.) Wettst-derived nanovesicles with anti-neuroblastoma potential

Edible plant-derived nanovesicles (PDNVs) have emerged as promising nanotherapeutic strategies for various diseases, including cancer. The biochemical composition and functional properties of PDNVs vary considerably on the basis of their botanical source. Bacopa monnieri (L.) Wettst is a medicinal plant renowned for its rich phytochemical profile, yet the isolation and biological activities of B. monnieri -derived nanovesicles (BMNVs) remain unexplored. We report, for the first time, the isolation, molecular cargo profiling, and in vitro functional evaluation of BMNVs against neuroblastoma cells. The isolated BMNVs displayed a characteristic bilayer morphology with an average particle size of [~]112 nm. Mass spectrometry-based metabolite analysis revealed an enrichment of triterpenoids and triterpene saponins, whereas protein cargo analysis revealed superoxide dismutase, which is correlated with their intrinsic free radical scavenging activity. In vitro assays demonstrated that BMNVs significantly suppress neuroblastoma cell growth and induce morphological alterations. Confocal three-dimensional reconstruction confirmed the cellular internalization of the BMNVs, revealing a distinct perinuclear distribution. This study provides the first evidence of the use of BMNVs as bioactive carriers, highlighting their potential as novel nanotherapeutic agents and establishing B. monnieri as a valuable natural resource for the development of bioactive plant-derived nanovesicles for nanomedicine. HighlightsO_LIFirst isolation and biophysical characterization of Bacopa monnieri-derived nanovesicles (BMNVs). C_LIO_LIBMNVs have diverse metabolite profiles and are notably enriched in triterpenoids and triterpene saponins. C_LIO_LIThe superoxide dismutase (SOD) identified within BMNVs confers intrinsic free radical-scavenging activity. C_LIO_LIBMNVs exhibit therapeutic potential as anti-neuroblastoma agents. C_LIO_LIThese edible plant-derived nanovesicles offer a versatile, biogenic platform to explore for further development in diverse therapeutic and nutraceutical applications. C_LI

pharmacology and toxicology↗

bioRxiv2022

Remodelled Ribosomes Synthesise a Specific Proteome in Proliferating Plant Tissue during Cold

Plant acclimation to low temperatures occurs through system-wide mechanisms that include proteome shifts, some of which occur at the level of protein synthesis. All proteins are synthesised by ribosomes. Rather than being monolithic, transcript-to-protein translation machines, ribosomes can be selective and cause effective proteome shifts required for successful temperature acclimation. Here, we use apical root meristems of germinating seedlings of the monocotyledonous plant barley as a model to study changes in protein abundance and synthesis rates during cold acclimation. We measure metabolic and physiological parameters that allow us to compare protein synthesis rates in different physiological states, e.g., in cold acclimation compared to the optimal temperature state. We show that ribosomal proteins are independently synthesised and assembled into ribosomal complexes in root proliferative tissue, and assess how the ribo-proteome shifts during cold may be associated with changes in synthesis and accumulation of macromolecular complexes. We demonstrate that translation initiation is the limiting step during cold acclimation and based on our data propose a model of a ribosomal code that depends on a reconfigured ribosome population, where as a mode of cold acclimation, specific ribosomal protein compositions may confer selective association capabilities between 60S subunits and 48S initiation complexes.

plant biology↗

bioRxiv2022

Effects of chitin and chitosan on root growth, biochemical defense response and exudate proteome of Cannabis sativa

Fungal pathogens pose a major threat to Cannabis sativa production, requiring safe and effective management procedures to control disease. Chitin and chitosan are natural molecules that elicit plant defense responses. Investigation of their effects on C. sativa will advance understanding of plant responses towards elicitors and provide a potential pathway to enhance plant resistance against diseases. Plants were grown in the in vitro Root-TRAPR system and treated with colloidal chitin and chitosan. Plant morphology was monitored, then plant tissues and exudates were collected for enzymatic activity assays, phytohormone quantification, qPCR analysis and proteomics profiling. Chitosan treatments showed increased total chitinase activity and expression of pathogenesis-related (PR) genes by 3-5 times in the root tissues. In the exudates, total peroxidase and chitinase activities and levels of defense proteins such as PR protein 1 and endochitinase 2 were increased. Shoot development was unaffected, but root development was inhibited after chitosan exposure. No significant effects on plant defense were observed upon chitin treatment. These results indicate that colloidal chitosan significantly promoted production and secretion of plant defense proteins in C. sativa root system and could be used as a potential elicitor, particularly in hydroponic scenarios to manage crop diseases. HighlightChitosan induces defense protein productions and secretions in the root tissues and exudates of C. sativa, offering a potential pathway to enhance plant resistance against fungal attack.

plant biology↗

bioRxiv2019

Evaluation of extraction solvents for untargeted metabolomics analysis of enrichment reactor cultures performing enhanced biological phosphorus removal (EBPR)

IntroductionThe extraction solvent mixtures were optimized for untargeted metabolomics analysis of microbial communities from two laboratory scale activated sludge reactors performing enhanced biological phosphorus removal (EBPR).\n\nObjectiveTo develop a robust and simple analytical protocol to analyse microbial metabolomics from EBPR bioreactors.\n\nMethodsExtra- and intra-cellular metabolites were extracted using five methods and analysed by ultraperformance liquid chromatography mass spectrometry (UPLC-MS).\n\nResultsThe optimal extraction method was biomass specific and methanol:water (1:1 v/v) and methanol:chloroform:water (2:2:1 v/v) were chosen, respectively, for each of the two different bioreactors.\n\nConclusionOur approach provides direct surveys of the metabolic state of PAO-enriched EBPR communities, showing that extraction methods should be carefully tailored to the microbial community under study

systems biology↗