Biology subjects

Stowe, E. J.

Publications and source records attributed to Stowe, E. J..

3 recordsLinked to original sources

bioRxiv2026

Regulators of ECM Structure Enable Functional Adaptation to Tensile Loading in Tendon Explants

Extracellular matrix (ECM) remodeling is essential for adaptation to changing mechanical demands, yet the mechanisms linking altered strain to functional outcomes remain poorly understood. This study aimed to define molecular and cellular programs driving the adaptation of tendon to increased (exercise) and decreased (disuse) strain. Male murine flexor tendon explants were cultured in incubator-housed tensile bioreactors and subjected to step changes in cyclic strain. After acclimation at 1% cyclic strain, exercise tendons experienced a step increase to 5% strain, while disuse tendons underwent stress deprivation. Increased strain produced significant mechanical adaptations, including increased elastic modulus and failure stress. Multiscale analyses of matrix organization, tissue composition, protein synthesis, signaling factors, and proteolytic activity revealed the mechanisms underlying these adaptations. Exercise-induced functional improvements were linked to an anabolic remodeling program characterized by TGF-{beta} and IL-6 signaling, small leucine-rich proteoglycan expression, MMP suppression, and enhanced collagen alignment. These findings indicate that regulators of matrix organization and turnover, beyond synthesis alone, are critical for functional adaptation. In contrast, mechanical unloading reduced collagen synthesis and alignment and promoted an MMP-dominant, catabolic phenotype favoring matrix breakdown. This study provides a comprehensive characterization of ECM remodeling, linking defined mechanical perturbations to molecular regulation and emergent structure-function relationships. These findings identify targetable mediators of adaptive remodeling and establish a framework for future studies of maladaptive ECM changes in aging, injury, and disease.

bioengineering↗

bioRxiv2024

Aged Tendons Exhibit Altered Mechanisms of Strain-Dependent Extracellular Matrix Remodeling

Aging is a primary risk factor for degenerative tendon injuries, yet the etiology and progression of this degeneration is poorly understood. While aged tendons have innate cellular differences that support a reduced ability to maintain mechanical tissue homeostasis, the response of aged tendons to altered levels of mechanical loading has not yet been studied. To address this question, we subjected young and aged murine flexor tendon explants to various levels of in vitro tensile strain. We first compared the effect of static and cyclic strain on matrix remodeling in young tendons, finding that cyclic strain is optimal for studying remodeling in vitro. We then investigated the remodeling response of young and aged tendon explants after 7 days of varied mechanical stimulus (stress-deprivation, 1%, 3%, 5%, or 7% cyclic strain) via assessment of tissue composition, biosynthetic capacity, and degradation profiles. We hypothesized that aged tendons would show muted adaptive responses to changes in tensile strain and exhibit a shifted mechanical setpoint, at which the remodeling balance is optimal. Interestingly, we found 1% cyclic strain best maintains native physiology while promoting ECM turnover for both age groups. However, aged tendons display fewer strain-dependent changes, suggesting a reduced ability to adapt to altered levels of mechanical loading. This work has significant impact in understanding the regulation of tissue homeostasis in aged tendons, which can inform clinical rehabilitation strategies for treating elderly patients.

bioengineering↗

bioRxiv2023

Cellular Senescence Impairs Tendon Extracellular Matrix Remodeling in Response to Mechanical Unloading

Musculoskeletal injuries, including tendinopathies, present a significant clinical burden for aging populations. While the biological drivers of age-related declines in tendon function are poorly understood, it is well accepted that dysregulation of extracellular matrix (ECM) remodeling plays a role in chronic tendon degeneration. Senescent cells, which have been associated with multiple degenerative pathologies in musculoskeletal tissues, secrete a highly pro-inflammatory senescence-associated secretory phenotype (SASP) that has potential to promote ECM breakdown. However, the role of senescent cells in the dysregulation of tendon ECM homeostasis is largely unknown. To assess this directly, we developed an in vitro model of induced cellular senescence in murine tendon explants. This novel technique enables us to study the isolated interactions of senescent cells and their native ECM without interference from age-related systemic changes. We document multiple biomarkers of cellular senescence in induced tendon explants including cell cycle arrest, apoptosis resistance, and SASP production. We then utilize this in vitro senescence model to compare the ECM remodeling response of young, naturally aged, and senescent tendons to an altered mechanical stimulus. We found that both senescence and aging independently led to alterations in ECM-related gene expression, reductions in protein synthesis, and tissue compositional changes. Furthermore, MMP activity was sustained, thus shifting the remodeling balance of aged and senescent tissues towards degradation over production. Together, this demonstrates that cellular senescence plays a role in the altered mechano-response of aged tendons and likely contributes to poor clinical outcomes in aging populations.

bioengineering↗