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Biology subjects

Steindl, S.

Publications and source records attributed to Steindl, S..

2 recordsLinked to original sources

Automation of tree-ring detection and measurements using deep learning

O_LIWe present an implementable neural network-based automated detection and measurement of tree-ring boundaries from coniferous species. C_LIO_LIWe trained our Mask R-CNN extensively on over 8,000 manually annotated rings. We assessed the performance of the trained model from our core processing pipeline on real world data. C_LIO_LIThe CNN performed well, recognizing over 99% of ring boundaries (precision) and a recall value of 95% when tested on real world data. Additionally, we have implemented automatic measurements based on minimum distance between rings. With minimal editing for missed ring detections, these measurements were a 99% match with human measurements of the same samples. C_LIO_LIOur CNN is readily deployable through a Docker container and requires only basic command line skills. Application outputs include editable annotations which facilitate the efficient generation of ring-width measurements from tree-ring samples, an important source of environmental data. C_LI

ecology↗

Multi-GBS: A massively multiplexed GBS-based protocol optimized for large, repetitive conifer genomes

Reduced representation library approaches are still a valuable tool for breeding and population and ecological genomics, even with impressive increases in sequencing capacity in recent years. Unfortunately, current approaches only allow for multiplexing up to 384 samples. To take advantage of increased sequencing capacity, we present Multi-GBS, a massively multiplexable extension to Genotyping-by-Sequencing that is also optimized for large conifer genomes. In Norway Spruce, a highly repetitive 20Gbp diploid genome with high population genetic variation, we call over a million variants in 32 genotypes from three populations, two natural forest in the Alps and Bohemian Alps, and a managed population from southeastern Austria using the existing TASSEL GBSv2 pipeline. Metric MDS analysis of replicated genotypes shows that technical bias in resulting genotype calling is minimal and that populations cluster in biologically meaningful ways.

genomics↗