Spangenberg, G. | Search bioRxiv

bioRxiv2020

A New and Improved Genome Sequence of Cannabis sativa

Cannabis is a diploid species (2n = 20), the estimated haploid genome sizes of the female and male plants using flow cytometry are 818 and 843 Mb respectively. Although the genome of Cannabis has been sequenced (from hemp, wild and high-THC strains), all assemblies have significant gaps. In addition, there are inconsistencies in the chromosome numbering which limits their use. A new comprehensive draft genome sequence assembly (~900 Mb) has been generated from the medicinal cannabis strain Cannbio-2, that produces a balanced ratio of cannabidiol and delta-9-tetrahydrocannabinol using long-read sequencing. The assembly was subsequently analysed for completeness by ordering the contigs into chromosome-scale pseudomolecules using a reference genome assembly approach, annotated and compared to other existing reference genome assemblies. The Cannbio-2 genome sequence assembly was found to be the most complete genome sequence available based on nucleotides assembled and BUSCO evaluation in Cannabis sativa with a comprehensive genome annotation. The new draft genome sequence is an advancement in Cannabis genomics permitting pan-genome analysis, genomic selection as well as genome editing.

genomics↗

bioRxiv2020

Automated Machine Learning for High-Throughput Image-Based Plant Phenotyping

Automated machine learning (AutoML) has been heralded as the next wave in artificial intelligence with its promise to deliver high performance end-to-end machine learning pipelines with minimal effort from the user. AutoML with neural architecture search which searches for the best neural network architectures in deep learning has delivered state-of-the-art performance in computer vision tasks such as image classification and object detection. Using wheat lodging assessment with UAV imagery as an example, we compared the performance of an open-source AutoML framework, AutoKeras in image classification and regression tasks to transfer learning using modern convolutional neural network (CNN) architectures pretrained on the ImageNet dataset. For image classification, transfer learning with Xception and DenseNet-201 achieved best classification accuracy of 93.2%, whereas Autokeras had 92.4% accuracy. For image regression, transfer learning with DenseNet-201 had the best performance (R2=0.8303, RMSE=9.55, MAE=7.03, MAPE=12.54%), followed closely by AutoKeras (R2=0.8273, RMSE=10.65, MAE=8.24, MAPE=13.87%). Interestingly, in both tasks, AutoKeras generated compact CNN models with up to 40-fold faster inference times compared to the pretrained CNNs. The merits and drawbacks of AutoML compared to transfer learning for image-based plant phenotyping are discussed.

plant biology↗