Biology subjects

Skrzypczak, T.

Publications and source records attributed to Skrzypczak, T..

3 recordsLinked to original sources

bioRxiv2026

Waking the sleepers: lincRNA overexpression compromises DHX36 activity and global protein synthesis

Transposable element-derived long intergenic noncoding RNAs (lincRNAs) are increasingly recognized as context-dependent regulators of gene expression; however, the functional consequences of their ectopic activation in somatic cells remain poorly understood. We previously showed that U7 snRNA represses a subset of LTR12-associated lincRNAs, including lnc-ARRDC4-1 and lnc-ADCYAP1-2, two testis-enriched lincRNAs with minimal expression in somatic cells. Here, we examined the consequences of their increased expression in somatic cells. We showed that overexpression of either lincRNA led to common transcriptomic changes, proteomic changes, impaired migration, altered adhesion and proliferation, and a ~50% reduction in protein synthesis. Furthermore, we identified lnc-ARRDC4-1 as an upstream regulator of lnc-ADCYAP1-2 transcription. Downstream of this event, lnc-ADCYAP1-2 interacts with the RNA helicase DHX36, a regulator of G-quadruplex-containing mRNAs. lnc-ADCYAP1-2 activation reduces DHX36 protein levels, which is accompanied by decreased protein output from a subset of DHX36 mRNA targets. At the cellular level, these effects correlate with cellular disfunction and altered global translation. Our results suggest a lnc-ARRDC4-1:lnc-ADCYAP1-2:DHX36 regulatory cascade linking the derepression of LTR12-containing lincRNAs to reduced protein synthesis and altered cellular processes in somatic cells.

Molecular Biology↗

bioRxiv2024

Altered viscoelastic properties of the Nicotiana tabacum BY-2 suspension cell lines adapted to high concentrations of NaCl and mannitol

To survive and grow, plant cells must regulate the properties of their cellular microenvironment in response to ever changing external factors. How the biomechanical balance across the cells internal structures is established and maintained during environmental variations remains a nurturing question. To provide insight into this issue we used two micro-mechanical imaging techniques, namely Brillouin light scattering and BODIPY-based molecular rotors Fluorescence Lifetime Imaging, to study Nicotiana tabacum suspension BY-2 cells long-term adapted to high concentrations of NaCl and mannitol. We discuss our results in terms of molecular crowding in cytoplasm and vacuoles, as well as tension in plasma membrane. The viscoelastic behavior was elucidated relative to cells external environments revealing the difference between the responses of cytoplasm and vacuole in the adapted cells. To understand how sudden changes in osmolarity affect cellular mechanics, the response of control and already adapted cells to further short-term osmotic stimulus was also examined. The applied correlative approach provides evidence that adaptation to hyperosmotic stress leads to different ratios of protoplast and environmental qualities that help to maintain cell integrity. Presented results demonstrate that the viscoelastic properties of protoplasts are an element of plant cells adaptation to high osmolarity.

plant biology↗

bioRxiv2024

Heterogenous transcriptomes of Nicotiana tabacum BY-2 suspension cell lines adapted to various osmoticum

Plants abiotic stress response and adaptation belong to the most important subjects in plants biology. Here, we present Nicotiana tabacum suspension cell lines adapted during long term cultures to high concentrations of NaCl, KCl, mannitol and sorbitol. Obtained lines differ in osmotic stress agents and final media osmolarities. RNA-seq analysis revealed similarities, as well as differences in adapted lines transcriptomes. Presented here BY-2 cells lines form a good model to reveal molecular mechanisms of plants adaptations to salt and osmotic stress on cellular level.

plant biology↗