Sjofjan, O. | Search bioRxiv

bioRxiv2023

Computational Insight into Anti-Obesity Effects of Combined Some Phytobiotics to GLP1R (Glucagon-Like Peptide 1 Receptor) Protein in Anas javanica

Damayanti CA, Harahap MAY, Wibowo S, Sjofjan O, Djunaidi IH. 2023. Computational Insight into Anti-Obesity Effects of Indonesian Phytobiotics to GLP1R (Glucagon-Like Peptide 1 Receptor) Protein in Anas javanica. Mojosari ducks (Anas javanica) is native Indonesia laying ducks was a egg producing type with quite high egg production, must be maintain body weight to propotional condition as laying duck. If the body weight surpasses normal, it can lead to obesity and reduce the eggs quality. One of the proteins closely related to obesity and hyperglycemia is GLP1R (Glucagon-Like Peptide 1 Receptor). The increase in GLP1R activity by one of the compounds that have been widely researched is loureirin B. Interaction between loureirin B and GLP1R increases insulin production in the body so that hyperglycemia and body weight can be controlled properly. Exploration of phytobiotic compounds from Indonesia is needed to find the substitution of loureirin B as an anti-obesity agent. According to the findings of in silico study (protein modeling and molecular docking), cynaroside (-9.2 kcal/mol), 14-Deoxy-11,12-didehydroandrographolide (-9.1 kcal/mol), rutin (-8.8 kcal/mol), andrographidine E (-8.6 kcal/mol), and cianidanol (-7.8 kcal/mol) had stronger binding affinity than loureirin B (-7.4 kcal/mol). Andrographidine E, derived from the plant Andrographis paniculata, is the best candidate for GLP1R agonist. The binding affinity that Andrographidine E has is lower than control compounds, so it is easier for bonds to occur between proteins and such compounds. In addition, the interacting amino acids do not have unfavourable bonds that make it more stable than other candidates. Results from clinical studies show that the use of A. paniculata can reduce glucose levels.

bioinformatics↗

bioRxiv2023

Anti-Inflammatory Potential of Selected Indonesian Phytogenic Blends to Nitric Oxide Synthase Inducible Protein in Mojosari Ducks (Anas javanica): In-silico Study

Inflammation is a defensive response to tissue damage, infectious agents, and injury. Necrotic enteritis is an inflammatory response induced by pathogenic bacteria invading the intestines of Mojosari ducks (Anas javanica). In contrast, excessive nitric oxide production by inducible nitric oxide synthase during inflammatory processes can cause significant intestinal tissue damage and cellular toxicity. Oxyresveratrol is an active compound of Morus alba that has been known to have antioxidant activity and can suppress the inflammatory process, inhibiting the increased expression of nitric oxide synthase (iNOS). However, unfortunately, this plant is not endemic to Indonesia, so using native Indonesian spices that can be a substitute for oxyresveratrol is necessary. The docking results from nine Indonesian phytogenic blends interacting with NOS revealed that cynaroside from the Piper betle L. plant might be utilized instead of oxyresveratrol as an anti-inflammatory drug via the inhibitory pathway of nitric oxide synthase protein. The docking results showed that from the nine compounds tested, it can be concluded that three compounds were found that are better than the control compound (oxyresveratrol) in terms of binding affinity (energy) and the type of hydrogen bonds bond in amino acid proteins that are equal to the amount more than all compounds tested. The three compounds are cynaroside compounds from Piper betle L. with a binding energy of -9.4 kcal / mol and a Conventional Hydrogen bond type GLU(B):761, GLU(A):761, curcumin compounds from Curcuma longa L. with a binding energy of -8.6 kcal/mol and Conventional Hydrogen bond type GLN(A):760, GLN(B):760 and compound 14-deoxy-11, 12-didehydroandrographolide from Andrographis paniculata with binding energy -8.8 kcal/mol and Conventional Hydrogen bond type GLU(B):761 can be used instead of oxyresveratrol as an anti-inflammatory agent through the inhibition pathway of Nitric Oxide Synthase protein (NOS)

biochemistry↗