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Biology subjects

Silva, J. P.

Publications and source records attributed to Silva, J. P..

2 recordsLinked to original sources

Multi-Temporal Remote Sensing of Inland Surface Waters: A Fusion of Sentinel-1 Data Applied to Small Seasonal Ponds in Semiarid Environments

Inland freshwater resources in semiarid environments play a key role in maintaining ecological systems and supporting human development. Space-based remote sensing spatiotemporal data have emerged as a new paradigm for understanding ecohydrological processes and trends, particularly in water-stressed areas. However, comprehensive cataloging is still lacking, especially in semi-arid regions and for small-sized water bodies (i.e., ponds), which are often overlooked despite their ecological relevance. In this study, high-resolution optical and radar Sentinel data (Sentinel-1 and Sentinel-2) were used to construct Sentinel-1&2-based local surface water (SLSW) models, to infer surface water occurrence and extent. To assess the reliability of this model, the results were compared with verification data, and separately with Landsat-based global water (LGSW) models. Three distinct semiarid regions were selected in SW Iberia, within a Mediterranean climate, each encompassing special protection areas for conservation and subjected to marked seasonality and bioclimatic changes. Surface water attributes were modeled using Random Forests for SLSW time series forecasting, which included the period from January 1, 2020, to December 31, 2021. During this period, the completeness of the archived information was compared between SLSW and LGSW, considering both intra-annual and inter-annual variations. The predictive performance of these models was then compared for specific periods (dry and wet), and each was independently validated with verification data. The results showed that SLWM achieved satisfactory predictive performances in detecting surface water occurrence ({approx}72%), with far greater completeness and reconstructed seasonality patterns compared to LGSW. The relatedness between SLSW and LGSW was stronger during wet periods (R2=0.38) than dry periods (R2=0.05), and SLSW related much better with the verification data (R2=0.66) than when compared to LGSW (R2=0.24). The proposed SLSW approach may therefore provide advantages in the delineation of dynamic surface water characteristics (occurrence and extent) in very small-sized water bodies (i.e., <0.5 ha), allowing for uninterrupted surface water time series forecasting at high spatiotemporal detail, and over extensive areas. Given the water constraints in semiarid regions and water resources vulnerability to climate change, our results show high potential for supporting a variety of activities underlying rural development and biodiversity conservation. Additionally, the socio-ecological applications of this research may help identify surface water anomalies (e.g., drought events) and enhance sustainable water supply governance, a particular priority in climate change hotspots. HighlightsO_LISurface water occurrence and extent was modeled across three semiarid regions C_LIO_LISentinel-1&2 data was compared with Landsat for characterizing very small water bodies C_LIO_LIModels based on Sentinel-1&2 resulted in a satisfactory classification precision C_LIO_LIVery high series completeness across seasons was found using Sentinel-1&2 data C_LIO_LISentinel-1&2 data was more reliable than Landsat when compared to verification data C_LI

ecology↗

Integrated Computational Biophysics approach for Drug Discovery against Nipah Virus

The Nipah virus (NiV) poses a pressing global threat to public health due to its high mortality rate, multiple modes of transmission, and lack of effective treatments. NiV glycoprotein G (NiV-G) emerges as a promising target for NiV drug discovery due to its essential role in viral entry and membrane fusion. Therefore, in this study we applied an integrated computational and biophysics approach to identify potential inhibitors of NiV-G within a curated dataset of Peruvian phytochemicals. Our virtual screening results indicated that these compounds could represent a natural source of potential NiV-G inhibitors with {Delta}G values ranging from -8 to -11 kcal/mol. Among them, Procyanidin B2, B3, B7, and C1 exhibited the highest binding affinities and formed the most molecular interactions with NiV-G. Molecular dynamics simulations revealed the induced-fit mechanism of NiV-G pocket interaction with these procyanidins, primarily driven by its hydrophobic nature. Non-equilibrium free energy calculations were employed to determine binding affinities, highlighting Procyanidin B3 and B2 as the ligands with the most substantial interactions. Overall, this work underscores the potential of Peruvian phytochemicals, particularly procyanidins B2, B3, B7, and C1, as lead compounds for developing anti-NiV drugs through an integrated computational biophysics approach. Key pointsO_LINipah Virus (NiV) Threat: NiV is a severe public health risk due to its high mortality rate, broad host range, multiple transmission modes, and lack of effective treatment. Outbreaks have occurred frequently in South and Southeast Asia, particularly in Bangladesh and India, leading to high fatality rates. C_LIO_LICross-Border Concerns: NiVs ability to transmit between humans and domestic animals raises concerns about its potential to cross regional borders and cause pandemics. It has been recognized as a high-priority pathogen by the World Health Organization. C_LIO_LILack of Treatment: Currently, there are no approved specific antiviral treatments or vaccines for NiV. Patients receive supportive care and some drugs used for other viruses, despite their side effects. C_LIO_LITargeting NiV Glycoprotein G: The study focuses on NiV glycoprotein G (NiV-G) as a target for potential anti-Nipah drugs due to its crucial role in viral entry. This glycoprotein mediates viral attachment and entry into host cells. C_LIO_LIComputational Drug Discovery: The research employs computational methods, including virtual screening and molecular dynamics simulations, to identify potential inhibitors of NiV-G from a dataset of Peruvian phytochemicals, particularly procyanidins B2, B3, B7, and C1. These compounds showed promising binding affinities, stable interactions, and favorable binding energies with NiV-G, making them potential lead compounds for drug development. C_LI

microbiology↗