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Sierra-Mejia, A.

Publications and source records attributed to Sierra-Mejia, A..

2 recordsLinked to original sources

Munger black raspberry: Insights into its role as a virus indicator for Rubus species

Munger black raspberry (Rubus occidentalis) has been a preferred indicator for Rubus viruses. The working hypothesis behind the Munger elevated susceptibility to viruses is its ability to sustain elevated virus titers; however, no research has yet explored this concept. To address this, we utilized an infectious clone of blackberry chlorotic ringspot virus to study the differences in virus expression between two Rubus species: R. occidentalis ( Munger) and R. L. subgenus Rubus Watson (blackberry, Natchez). Our data demonstrate that virus accumulation in Munger is over 47,300 times higher compared to Natchez, enhancing our understanding of virus-host dynamics and providing valuable insights into why Munger is a preferred indicator species for virus detection in Rubus spp.

plant biology↗

Overcoming the Woody Barrier: Dodder Enables Efficient Transfer of Infectious Clones to Woody Plants

Woody hosts are notoriously resistant to genetic transformation. Traditional methods, such as Agrobacterium-mediated transformation, are often inefficient, and this limitation extends to delivering infectious clones to woody plants. Dodder species (Cuscuta spp.) are holoparasitic plants that can establish direct connections with the vascular tissue of the parasitized plants, allowing them to facilitate virus transmission between unrelated botanical species. We demonstrated that a novel dodder-based approach achieved superior transmission in Rubus spp. compared to direct agroinoculation. The transmission rates for systemic blackberry chlorotic ringspot virus transmission increased from 9% to 73%, whereas the transmission of the phloem-restricted blackberry yellow vein associated virus rose from 0% to 46%. This novel method expands the toolbox available to plant biologists to study virus-host interactions in woody plants.

plant biology↗