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Biology subjects

Shome, A.

Publications and source records attributed to Shome, A..

2 recordsLinked to original sources

Assessing the Incidence and Severity of Field Diseases in Marigold (Tagetes erecta) Cultivation: Results from Jashore District, Bangladesh

A research investigation was carried out in the Jashore district of Bangladesh to study diseases affecting marigold plants, while the laboratory analyses were conducted in the Plant Disease Study Facility, Sher-e-Bangla Agricultural University, Dhaka. The experiment was conducted by surveying three farmers from each of the sixteen villages across four unions in Jhikargacha Upazila to document the occurrence and severity of diseases under natural field conditions. The fungal pathogens surveyed in these diseases were six: leaf spot, botrytis blight, flower bud rot, stem rot, foliage blight, white mold-soil-borne diseases. The most important microflora reported to be associated with these diseases include Alternaria alternata, Botrytis cinerea, Alternaria dianthi, Fusarium oxysporum, Curvularia lunata, and Sclerotinia sclerotiorum. Of these, major diseases included leaf spot, botrytis blight, foliage blight or flower and leaf blight, and flower bud rot, while minor diseases included stem rot and white mold. Disease incidence ranged from 0-54.33%, while disease severity varied from 1-24%. The paper is the first record of marigold pathogens in Bangladesh and will be highly useful during disease identification, and it would lead to the better cultivation of marigolds in this area.

plant biology↗

Optimization of conditions for conjugation of outer membrane vesicles of Salmonella Typhimurium with oral delivery systems using response surface method

BackgroundOuter membrane vesicles (OMVs) released from Salmonella Typhimurium can become the effective vaccine candidate. For efficient delivery through oral route, the OMVs are conjugated with some delivery systems. The current study was aimed at optimization of conditions required for conjugation of OMVs with nano- or microparticles for maximum entrapment of OMV in terms of protein concentration. MethodsThe OMVs of Salmonella Typhimurium were conjugated under three optimum conditions of pH, temperature and ratio (nanoparticles or microparticles: OMVs) predicted by response surface method. The efficiency of conjugation was determined by entrapment of OMVs in nano/microparticles. ResultThe pH and temperature were not influential conditions in case of conjugation of OMVs with chitosan nanoparticles (Ch-NP) and poly-lactide co-glycolide microparticles (PLG-MP) while they had influence in case of poly(anhydride) nanoparticles. In case of Ch-NP and PLG-MP, the optimum ratio for maximum entrapment was found to be 1:10 and 1:9 respectively. The optimum pH and temperature was found to be 7 and 24{degrees}C respectively for conjugation of poly(anhydride) nanoparticles. The optimized conditions did not alter the protein profile and immunogenic potential of conjugated vaccines.

biochemistry↗