Sham, L. T. | Search bioRxiv

bioRxiv2026

The WalRK two-component system in Streptococcus pneumoniae ensures robustness of secondary wall polymer attachment

Capsular polysaccharide (CPS) is essential for Streptococcus pneumoniae virulence. Yet, the mechanism linking CPS to peptidoglycan (PG) remains unclear. Here, we identified a strong negative genetic interaction between the genes encoding the putative capsule ligase CpsA and the WalK histidine kinase, a component of the WalRK two-component system regulating cell wall homeostasis. In the absence of cpsA, capsule polymers compete with wall teichoic acids for ligase activity to PG. This induces cell wall stress and is sensed by the WalRK system. Overexpression of the PG hydrolase pcsB or disruption of the PG-modifying enzymes pgdA and oatA restored growth of strains lacking cpsA and walK. Furthermore, CpsA overproduction compensates for the loss of other LytR-Cps2A-Psr (LCP) ligases, suggesting it can support capsule and wall teichoic acid syntheses. These findings support the model that LCP ligases are semi-redundant, although they may install secondary polymers on a different residue of PG. This work also suggests that WalRK signaling compensates for reduced capsule and WTA attachment by positively regulating PG hydrolases. SIGNIFICANT STATEMENTStreptococcus pneumoniae causes approximately half a million deaths annually. A powerful public health tool for controlling pneumococcal infections is vaccination against the protective capsule. Yet, the mechanisms by which the capsule layer attaches to the underlying cell wall remain poorly defined. This study shows that the conserved capsule gene CpsA is not strictly required for capsule attachment but instead works together with other LytRIZCpsAIZPsr (LCP) ligases. However, it requires the essential WalRK signaling system to maintain cell envelope integrity. Defects in LCP activity are alleviated by WalRKIZdriven upregulation of peptidoglycan hydrolases, overexpression of PcsB, or inactivating peptidoglycan modifications that limit hydrolysis. These findings reveal coordination among flux to capsule synthesis, secondary wall polymer attachment, and cell wall remodeling. TEASERThe WalRK two-component system responds to cell envelope stress caused by reduced LCP ligase activity.

microbiology↗

bioRxiv2024

Dual transposon sequencing (Dual Tn-seq) to probe genome-wide genetic interactions

Understanding gene function on a genome-wide scale is a fundamental goal in biology. With the advent of next-generation sequencing, high-throughput methods such as transposon sequencing (Tn- seq) were developed to measure fitness of single deletion mutants en masse. Nevertheless, gene redundancy complicates these approaches, as inactivating genes individually may not produce discernable phenotypes. Here, we report Dual Tn-seq, a technique for simultaneously assaying fitness of a comprehensive pool of double mutants. Dual Tn-seq couples random barcode transposon-site sequencing (RB Tn-seq) with the Cre-lox system, allowing us to determine the fitness of {approx}68% of all possible double mutant combinations in the human pathogen Streptococcus pneumoniae. Genetic interactions identified from {approx}1.4 billion double mutants uncovered new avenues in widely conserved biochemical pathways, exemplified by the discovery of a new CTP synthase and a regulator of peptidoglycan biosynthesis. Since Dual Tn-seq has very few requirements, it can be readily adapted to diverse organisms.

microbiology↗