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Biology subjects

Shah, S. M. A.

Publications and source records attributed to Shah, S. M. A..

2 recordsLinked to original sources

CitriBEiTNet: A Hybrid CNN-Transformer Architecture Combining MobileNetV2 with BEiT's Global Attention for Automated Citrus Leaf Disease Diagnosis

Citrus farming plays an essential role in agriculture; however, diseases like canker, greening, black spot, and melanose significantly reduce yield and fruit quality. Efficient classification of citrus leaf diseases is important for crop health maintenance and optimal crop yield. Traditional methods for leaf disease detection are slow, labor-intensive, and often inaccurate, which highlights the need for automated solutions. This research presents a novel hybrid approach for identifying citrus diseases by combining a vision transformer with deep learning architectures. Using Bidirectional Encoder Representation from Image Transformers (BEIT) and MobileNetV2 as feature extractors, the proposed model captures distinctive features from images, which are then classified using Support Vector Machine (SVM). The dataset includes four different disease categories and a healthy class. Data augmentation techniques are applied to improve model robustness. The experimental findings demonstrate that CitriBEiTNet achieves a remarkable training accuracy of 99.82% and a testing accuracy of 99.57%, outperforming current leading techniques. This model provides an efficient, scalable, and economical approach for early disease identification, enabling farmers to take preventive measures and improve agricultural yields.

bioengineering↗

Two TAL effectors of Xanthomonas citri pv. malvacearum target GhSWEET15 as the susceptibility genes for bacterial blight of cotton

Bacterial Blight of Cotton (BBC) caused by Xanthomonas citri pv. malvacearum (Xcm) is an important and destructive disease affecting cotton plants. Transcription activator-like effectors (TALEs) released by the pathogen regulate cotton resistance to the susceptibility. In this study, we sequenced the whole genome of Xcm Xss-V2-18 and identified eight tal genes; seven on the plasmids and one on the chromosome. Deletion and complementation experiments of Xss-V2-18 tal genes demonstrated that Tal1b is required for full virulence on cotton. Transcriptome profiling coupled with TALE-binding element prediction revealed that Tal1b targets GhSWEET15A04/D04 and GhSWEET15D02 simultaneously. Expression analysis confirmed the independent inducibility of GhSWEET15A04/D04 and GhSWEET15D02 by Tal1b, whereas GhSWEET15A04/D04 is additionally targeted by Tal1. Moreover, GUS ({beta}-glucuronidase) and Xa10-mediated HR (hypersensitive response) assays indicated that the EBEs are required for the direct and specific activation of the candidate targets by Tal1 and Ta1b. These findings may advance our understanding of the dynamics between TALEs and EBEs, and decipher a simple and effective DNA-binding mechanism that could lead to the development of more efficient methods for gene editing and transgenic research.

pathology↗