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Senapati, B.

Publications and source records attributed to Senapati, B..

3 recordsLinked to original sources

Hunger reconfigures a reward learning circuit into a memory competent mode

Internal states such as hunger dynamically reshape activity across circuits to support resource seeking. Neuromodulation provides a means of controlling such physiological properties of neurons, but how this flexibility regulates memory networks remains unclear. Here, we describe how hunger reconfigures a dopaminergic food-reward circuit between two physiological modes that support memory formation, in Drosophila. Starvation suppresses baseline dopaminergic activity through peptidergic signalling, enabling reward-evoked, large-amplitude dopamine neuron spikes to reinforce learning. This spiking mode can be engaged by sugar consumption and persists beyond feeding, reflecting the fly's satiety state. Persistent dopaminergic large-amplitude spiking reinforces learning and transitions the memory network into a mode that prioritizes consolidation of recently acquired memories. The transition from hunger to satiation is also reflected in the activity of postsynaptic output neurons that shift from a tonic, decorrelated mode that is responsive to dopamine into a bursting, correlated mode in which further dopaminergic modulation is occluded. Therefore, nutrient deprivation reconfigures the memory circuit into a learning competent dopamine-receptive mode, which persistent reinforcing dopamine then switches into a satiated mode driving memory consolidation. Together these processes mechanistically intertwine state-dependent reward-signalling with subsequent memory stabilization through transitions in physiological mode.

neuroscience↗

Visceral signaling of post-ingestive malaise directs memory updating in Drosophila

Consolidation is a time when labile memories transition to a stable form. Malaise learning in Drosophila reveals consolidation to also permit memory updating. Flies taught to associate one of two odors with toxin-tainted sugar initially express conditioned odor approach, that following consolidation switches to avoidance. Behavioral reversal emerges from dopaminergic update of parallel memories for the two trained odors. Differential serotoninergic modulation of specific aversive and rewarding dopaminergic neuron subtypes permits post-ingestive intoxication to suppress consolidation of initial odor-sugar memory and simultaneously invert reward memory plasticity into "safety" memory for the odor experienced without food. Fat body release of the Toll-ligand activating protease modSP, and resilience factor Turandot A, instruct malaise updates by triggering autocrine Toll signaling in the same brain dopaminergic neurons that form and consolidate initial sugar memory. This neural mechanism overcomes the credit assignment problem of delayed post-ingestive reinforcement by updating earlier memories of the trained odors.

neuroscience↗

AP-2α/AP-2β transcription factors are key regulators of epidermal homeostasis

AP-2 transcription factors regulate ectodermal development but their roles for epidermal homeostasis in the adult skin are unknown. We find that AP-2 is the predominant AP-2 family member in adult epidermis, followed by AP-2{beta}. Through inactivation of AP-2, AP-2{beta}, or both in keratinocytes we assessed the effects of a gradient of epidermal AP-2 activity on skin function. We find that (1) loss of AP-2{beta} in keratinocytes is compensated for by AP-2, (2) loss of AP-2 impairs terminal keratinocyte differentiation and hair morphogenesis, and (3) the combined loss of AP-2/AP-2{beta} results in more severe skin and hair abnormalities. Keratinocyte differentiation defects precede a progressive neutrophilic skin inflammation. Inducible inactivation of AP-2/AP-2{beta} in the adult phenocopies these manifestations. Transcriptomic analyses of epidermis lacking AP-2 or AP-2/AP-2{beta} in keratinocytes demonstrate a terminal keratinocyte differentiation defect with upregulation of alarmin keratins and of several immune pathway regulators. Moreover, our analyses suggest a key role of loss of AP-2-dependent gene expression of CXCL14 and KRT15 as an early pathogenic event towards the manifestation of skin inflammation. Thus, AP-2/AP-2{beta} are critical regulators of epidermal homeostasis in the adult skin.

cell biology↗