Biology subjects

Semyachkina-Glushkovskaya, O. V.

Publications and source records attributed to Semyachkina-Glushkovskaya, O. V..

4 recordsLinked to original sources

bioRxiv2021

Pilot identification of the Live-1/Prox-1 expressing lymphatic vessels and lymphatic elements in the unaffected and affected human brain

We report here a pilot identification of the presence of the lumenized Lyve-1/Prox-1-expressing vessels with distinct walls composed of a single endothelial layer in the unaffected brain and with intraventricular hemorrhages. These lymphatic vessels (LVs) have valves and an undulating shape in the distal region that is the classical characteristic of lymphatic precollectors. Furthermore, we identified Lyve-1/Prox-1-expressing lymphatic elements in the enlarged perivascular spaces. These pioneering results might be a revolutionary step in our understanding of anatomy and physiology of the cerebral lymphatics and stimulate a reassessment of basic assumptions in the aetiology of brain diseases associated with lymphatic dysfunction. The discovery of the cerebral LVs is a crucial step in the development of breakthrough technologies of modulations of lymphatic removing of toxins and blood from the brain. One Sentence SummaryLyve1/Prox1-expressing lymphatic vessels and elements in the unaffected and affected human brain.

neuroscience↗

bioRxiv2020

Photostimulation of lymphatic clearance of red blood cells from the mouse brain after intraventricular hemorrhage

Intraventricular hemorrhage (IVH) is the most fatal form of brain injury, yet a therapy directed at ameliorating intraventricular clot is very limited. There is accumulating evidence that an augmentation of the meningeal lymphatic (MLVs) functions might be a promising therapeutic target for IVH. In particular, the photostimulation (PS) of MLVs could be promising for non-invasive therapy of IVH via PS of clearance of red blood cells (RBCs) from the brain via MLVs. Indeed, we uncover that PS has therapeutic effects on IVH in mice reducing the mortality, improving the emotional status, accelerating the RBCs evacuation from the ventricles and increasing the ICP recovery. Our findings strongly suggest that the PS-mediated stimulation of drainage and clearing functions of MLVs can be a novel bedside, readily applicable and commercially viable technologies for treatment of IVH. These pilot results open new horizons in a non-invasive therapy of IVH via PS stimulation of regenerative lymphatic mechanisms.

neuroscience↗

bioRxiv2020

Phenomenon of music-induced opening of the blood-brain barrier in healthy mice

Music plays a more important role in our life than just being an entertainment. It is an even anti-anxiety therapy of human and animals. However, the unsafe listening of loud music triggers hearing loss in millions of young people and professional musicians (rock, jazz, and symphony orchestra) due to exposure to damaging levels of sound using personal audio devices or at noisy entertainment venues including nightclubs, discotheques, bars, and concerts. Therefore, it is important to understand how loud music affects us. In this pioneering study on healthy mice, we discover that loud rock music below the safety threshold causes opening of the blood-brain barrier (OBBB), which plays an important role in protecting the brain from viruses, bacteria and toxins. We clearly demonstrate that listening loud music during 2 hrs in an intermittent adaptive regime is accompanied by delayed (1h after music exposure) and short-lasting (during 1-4 hrs) OBBB to low and high molecular weight compounds without cochlear and brain impairments. We present the systemic and molecular mechanisms responsible for music-induced OBBB. Finally, a revision of our traditional knowledge about the BBB nature and the novel strategies in optimization of sound-mediated methods for brain drug delivery are discussed.

neuroscience↗

bioRxiv2020

Diffusion assessment through image processing: beyond the point-source paradigm

The quantification of transport processes of different substances in the brains parenchyma is important in the context of understanding brain functioning. Most of the currently used methods for assessment of the effective diffusion coefficient rely on the point-source paradigm. We propose a method for the quantitative characterization of the diffusion process in the brains parenchyma using a set of images recorded in the experiment during the spreading of a fluorescent dye. Our method exploits the frame-wise comparison of experimental data with a set of images that would be observed for an ideal diffusion process within the same topology. We obtain this reference set of images using blurring the image with an appropriate kernel function, and the degree of such blurring correlates with the spreading process of a dye. We demonstrate the applicability of the proposed method using (i) the simulated surrogate data, (ii) the set of experimentally recorded fluorescent images of the isolated event of blood-brain barrier (BBB) opening, and (iii) the images of massive multi-source spreading of fluorescent dye.

biophysics↗