Biology subjects

Schuster, F.

Publications and source records attributed to Schuster, F..

2 recordsLinked to original sources

bioRxiv2025

High Throughput Information Extraction of Printed Specimen Labels from Large-Scale Digitization of Entomological Collections using a Semi-Automated Pipeline

O_LINatural history museums curate billions of insect specimens, forming a vast but underutilized resource for biodiversity research. While digitization efforts have increased the availability of high-resolution specimen images, extracting metadata from labels remains a major bottleneck, often requiring manual transcription. C_LIO_LIWe developed a semi-automated pipeline, ELIE (Entomological Label Information Extraction), which combines computer vision, convolutional neural networks (CNNs), optical character recognition (OCR), and clustering algorithms to streamline label data extraction. Our pipeline operates in three stages: (1) label detection and classification (printed vs. handwritten), (2) OCR-based text extraction from printed labels using Tesseract or Google Vision, and (3) clustering of extracted text for human validation of outliers. C_LIO_LIBenchmarking on diverse datasets from multiple museum collections showed that our approach successfully extracted and clustered up to 98% of printed labels, significantly reducing manual effort. The pipeline improves efficiency in digitization workflows while maintaining high accuracy in label data capture. C_LIO_LIOur approach demonstrates the potential of integrating AI-driven methods with human validation to accelerate specimen digitization. By reducing manual transcription workload and enabling scalable extraction of insect label metadata, it unlocks biodiversity data for research in ecology, systematics, and conservation globally. C_LI

bioinformatics↗

bioRxiv2020

TMEM41B is a pan-flavivirus host factor

Flaviviruses pose a constant threat to human health. These RNA viruses are transmitted by the bite of infected mosquitoes and ticks and regularly cause outbreaks. To identify host factors required for flavivirus infection we performed full-genome loss of function CRISPR-Cas9 screens. Based on these results we focused our efforts on characterizing the roles that TMEM41B and VMP1 play in the virus replication cycle. Our mechanistic studies on TMEM41B revealed that all members of the Flaviviridae family that we tested require TMEM41B. We tested 12 additional virus families and found that SARS-CoV-2 of the Coronaviridae also required TMEM41B for infection. Remarkably, single nucleotide polymorphisms (SNPs) present at nearly twenty percent in East Asian populations reduce flavivirus infection. Based on our mechanistic studies we hypothesize that TMEM41B is recruited to flavivirus RNA replication complexes to facilitate membrane curvature, which creates a protected environment for viral genome replication. HIGHLIGHTSTMEM41B and VMP1 are required for both autophagy and flavivirus infection, however, autophagy is not required for flavivirus infection. TMEM41B associates with viral proteins and likely facilitates membrane remodeling to establish viral RNA replication complexes. TMEM41B single nucleotide polymorphisms (SNPs) present at nearly twenty percent in East Asian populations reduce flavivirus infection. TMEM41B-deficient cells display an exaggerated innate immune response upon high multiplicity flavivirus infection.

molecular biology↗