Biology subjects

Schulte, R.

Publications and source records attributed to Schulte, R..

2 recordsLinked to original sources

bioRxiv2017

A tale of two cores: a quantitative comparative analysis of the institutional and the open access models for flow research technology platforms

Quinquennium of sorting for Cambridge Quinquennium of 'sorting for... Emerging divergence: origins Shortage of capacity: perception... Current and projected capacity... Conclusions References Biomedical specialist core facilities are major part of institutional infrastructure in academia and play important role in research by creating access to well established and emerging technologies and being a source of valuable expertise. In each particular case, the extent, range and content of services they provide are determined by the technology specialism, demands of the users and institutional/overarching policies.\n\nIn 2012, two flow cytometry core facilities embarked on a new journey in development of their services to research community in Cambridge. Both facilities started from the same initial level: ...

scientific communication and education↗

bioRxiv2017

Heterogeneity of hypothalamic Pro-opiomelanocortin-expressing neurons revealed by single-cell RNA sequencing

Arcuate proopiomelanocortin (POMC) neurons are critical nodes in the control of body weight. Often characterised simply as direct targets for leptin, recent data suggest a more complex architecture. Using single cell RNA sequencing, we have generated an atlas of gene expression in murine POMC neurons. Of 163 neurons, 118 expressed high levels of Pomc with little/no Agrp expression and were considered \"canonical\" POMC neurons (P+). The other 45/163 expressed low levels of Pomc and high levels of Agrp (A+P+). Unbiased clustering analysis of P+ neurons revealed four different classes, each with distinct cell surface receptor gene expression profiles. Further, only 12% (14/118) of P+ neurons expressed the leptin receptor (Lepr) compared with 58% (26/45) of A+P+ neurons. In contrast, the insulin receptor (Insr) was expressed at similar frequency on P+ and A+P+ neurons (64% and 55%, respectively). These data reveal arcuate POMC neurons to be a highly heterogeneous population.

neuroscience↗