Biology subjects

Saka, D. K.

Publications and source records attributed to Saka, D. K..

3 recordsLinked to original sources

bioRxiv2024

ASSESSMENT OF THE IMPACT OF SLEEP DEPRIVATION AND DIET ON GROWTH, COGNITION, INFLAMMATION, OXIDATIVE STRESS, AND TISSUE HISTOLOGY

This study explores the interplay encompassing amino acids roles, metabolic processes, growth, and the intricate relationships between sleep patterns, amino acid-deficient diet, and caffeine consumption, with emphasis on the vital connections between diet, oxidative stress, and cognitive health while investigating the essential role of quality sleep in maintaining cognitive function. This study investigates the intricate connections among amino acids, metabolic processes, growth, sleep patterns, amino acid-deficient diets, and caffeine consumption. Emphasizing the crucial links between diet, oxidative stress, and cognitive health, the research underscores the essential role of quality sleep in maintaining cognitive function. The bidirectional relationship between disrupted sleep and cognitive health, as well as the impact of caffeine on cognitive function, is explored. The study involved adult male Wistar rats divided into 10 groups A - J based on cage conditions and diet. Group A-E were kept in a normal cage with diets containing varying levels of tryptophan and other essential amino acids, while Group F-J underwent sleep deprivation using a disk-over-water method and received diets with varying tryptophan and other essential amino acid levels, in addition to caffeine. The experiment spanned 2 weeks, blood samples were collected on days 1,4,7, and 13 for relevant biochemical serum analyses, and the rats were sacrificed on the fourteenth day for blood sample collection, biochemical assays, and brain histology. The 2-week experiment revealed a linear decrease in melatonin and serotonin levels across all caffeine-administered groups, possibly due to stress and anxiety as compared with the reduction in these parameters observed in control rats, suggesting melatonin and serotonin as potential sleep deprivation biomarkers. Testosterone and insulin-like growth hormone assays showed a significant decrease in sleep-deprived rats with caffeine, impacting secondary sex characteristics and growth. Elevated TNF- and interleukin-1b levels in the caffeine-administered, sleep-deprived group suggest potential health risks associated with sleep deprivation and caffeine. Serum assays for antioxidant enzymes indicated heightened oxidative stress in sleep-deprived rats with caffeine. Histopathological studies revealed cellular abnormalities in the sleep-deprived group, indicating increased cellular metabolism and potential pathological conditions. This study elucidates the complex relationships among sleep, dietary factors, and hormonal and inflammatory responses. It underscores the potential neurotoxic effects associated with caffeine consumption when coupled with sleep deprivation. These results underscore the urgent need for further investigation into dietary strategies that could alleviate these adverse effects and promote better health.

neuroscience↗

bioRxiv2024

Mitigation of Reproductive Function in Sodium Fluoride-administered Wistar Rats by Tamarindus indica Fruit Pulp Ethanol Extract

Infertility is a stigmatized and emotionally challenging issue for affected individuals, often compounded by the high costs of conventional fertility treatments. This study investigated the prophylactic and therapeutic effects of an ethanolic extract of Tamarindus indica fruit pulp on sodium fluoride-induced sexual and reproductive dysfunction in Wistar rats. The extract was prepared via ethanol maceration, followed by filtration and drying. Seventy Wistar rats were used, with an initial acute toxicity study indicating no adverse effects at 5000 mg/kg of the extract. Following toxicity assessment, rats were divided into seven groups, with varying treatments involving Tamarindus indica extract, sodium fluoride, and clomid. After the treatment period, biochemical analyses revealed that rats treated with 400 mg/kg of Tamarindus indica extract showed significant improvements (p < 0.05) in sperm count, luteinizing hormone (in males), testosterone, estrogen, along with reduced levels of follicle-stimulating hormone (in males), and luteinizing hormone (in females). Histopathological examination revealed normal histomorphology in certain groups treated with the extract. Phytochemical analysis identified the presence of tannins and steroids in the extract. The study concluded that Tamarindus indica fruit pulp extract exhibits both prophylactic and therapeutic effects on sodium fluoride-induced sexual and reproductive dysfunction in rats, with the most significant effects observed at a 400 mg/kg dosage. These findings suggest that Tamarindus indica extract could serve as an alternative to conventional treatments for infertility.

pharmacology and toxicology↗

bioRxiv2024

Amelioration of Oxidative Stress in Sodium Fluoride-administered Wistar Rats by Tamarindus indica Fruit Pulp Ethanolic Extract

Infertility is a significant global health issue often exacerbated by environmental toxins like sodium fluoride (NaF), which induces oxidative stress and reproductive dysfunction. Medicinal plants, such as Tamarindus indica, known for their antioxidant properties, offer potential natural remedies. This study evaluates the antioxidant potential of the ethanolic extract of Tamarindus indica fruit pulp in mitigating NaF-induced infertility in Wistar rats. Tamarindus indica fruits were authenticated and processed into a crude extract, with phytochemical screening revealing the presence of tannins and steroids. Seventy Wistar rats were divided into seven groups, receiving various treatments including T. indica extract, NaF, and Clomid. The acute toxicity test, conducted according to WHO and OECD guidelines, showed no lethal effects up to 5000 mg/kg of the extract. Phytochemical screening indicated that the antioxidant properties of the extract could be attributed to tannins and steroids. Experimental groups treated with T. indica extract exhibited significant improvements in oxidative stress markers such as malondialdehyde (MDA), glutathione peroxidase (GPx), glutathione (GSH), catalase (CAT), and superoxide dismutase (SOD) compared to control groups. The 200 mg/kg preventive group demonstrated the most notable antioxidant activity, comparable to the normal control group. The findings suggest that the ethanolic extract of Tamarindus indica fruit pulp at 200 mg/kg body weight has significant potential as a natural therapeutic agent against oxidative stress-induced infertility. This highlights its promise as an alternative to conventional treatments, offering a safe and effective option for managing reproductive health issues related to oxidative stress.

biochemistry↗