Biology subjects

Saheed, M. I.

Publications and source records attributed to Saheed, M. I..

3 recordsLinked to original sources

bioRxiv2021

Influence of selected plant growth stimulators in enhancing germinability and germination parameters of Zea mays L. under microgravity conditions simulated by a two-dimensional clinostat

The earth has become increasingly overcrowded as a result of rapid urbanization and population growth, predicting that its carrying capacity could be overstretched. As a result, it is important to test the possibilities of growing plants under space exploration conditions, especially gravitational balance. Since microgravity impedes plant development, to what extent can plant growth stimulators reverse or enhance this trend? A total of 12 maize seeds were weighed and placed sideways in petri dish and inoculated with plant growth stimulators such as indole acetic acid (IAA), gibberellic acid (GA), and ascorbate (AA) and the clinorotated at different rates (0.5, 1.0 and 2.0 rpm), while control seeds were just placed on a table. Results showed that at 72 hrs, the maize seeds under microgravity showed reduced germination percentage with increasing clinorotation rates as against the control. But when stimulated with IAA, GA and AA, improved germination percentage was observed as against the control even under microgravity condition. The seedling dry weight, germination time and other germination parameters also showed similar improvements. Comparatively, the three growth stimulators showed no major variations in their ability to improve germination percentage under microgravitational impact. However, IAA showed more improvement on seedling vigor as against others, while GA showed more effect on the peak time and rate of germination. This research confirmed the possibilities of improving germinability of maize seeds under space exploration condition.

plant biology↗

bioRxiv2021

Seed bio-priming with phosphate solubilizing bacteria strains to improve rice (Oryza sativa L. var. FARO 44) growth under ferruginous ultisol conditions

The research investigated the possibility of phosphate solubilizing bacteria (PSB) with plant growth-promoting (PGP) capabilities to improve growth properties of rice plant under ferruginous ultisol (FU) condition through bio-priming strategy. The PSB with PGP properties used in this research were Bacillus cereus strain GGBSU-1, Proteus mirabilis strain TL14-1 and Klebsiella variicola strain AUH-KAM-9 that were previously isolated and characterized following the 16S rRNA gene sequencing. Biosafety analysis of the PSB isolates was conducted using blood agar. The rice seeds were then bio-primed with the PSBs at 3, 12 and 24 hours priming durations and then sown in a composite FU soil sample. Differences in germination bioassay involving SEM, morphology, physiology and biomass parameters were investigated for 15 weeks after bio-priming. The composite FU soil used in the study had high pH, low bioavailable phosphorus, low water holding capacity and high iron levels which has led to a low growth properties of rice seeds without bio-priming in FU soil. Germination parameters was better in seeds bio-primed with the PSBs, especially at 12h priming duration as against seeds without priming. SEM showed more bacterial colonization in the PSB bio-primed seeds. Seed bio-priming of rice seed with Bacillus cereus strain GGBSU-1, Proteus mirabilis strain TL14-1 and Klebsiella variicola strain AUH-KAM-9 under FU soil condition significantly improved seed microbiome, rhizocolonization and soil nutrient properties, thereby enhancing growth properties of the rice plant. This suggest the ability of PSB to solubilize and mineralize soil phosphate and improve its availability and soil property for optimum plant usage in phosphate stressed and iron toxic soils.

plant biology↗

bioRxiv2020

Screening bacterial isolates for phosphate Solubilizing capability in a ferruginous ultisol in Benin City, Edo State Nigeria.

Phosphorus is a major growth-limiting nutrient which plays important biochemical role in photosynthesis, respiration and several other processes in the living plant. It is widely distributed in minerals as phosphates. It reacts easily with Fe3+ in ferruginous ultisols and therefore not bioavailable for plant usage. Many bacteria have the ability to solubilize phosphate minerals and make it bioavailable to plants. Thus this research investigates the culturable bacterial composition of ferruginous ultisol, comparative to control soils as well as the phosphate solubilizing capabilities of the isolates for future use in soil improvements. Six soil samples of different ferruginous levels and a control were assayed for physicochemical parameters prior to the experiment. Culturable bacteria as well as the phosphate solubilizing bacteria (PSB) were assayed in Pikovskayas medium at 27{degrees}C with 7.5 pH for 7days. Six distinct isolates were observed which proved to be Proteus spp., Pseudomonas spp., Klebsiella spp., Salmonella spp., Bacillus spp. and Serratia spp. based on biochemical and morphological characteristics. Of these six isolates, three isolates (EMBF2-Klebsiella spp, BCAF1- Proteus spp and BCAC2- Bacillus spp) were identify to solubilize phosphate by releasing a considerable amount of phosphate (12.01-21.23 ppm) and lowering the pH of the media. The three isolates showed tolerance to acidic and alkaline media and also showed plant growth promoting capabilities by releasing IAA and siderophores. The result revealed that the three isolates had potential to chelate the ion bond in Fe3+ in ferruginous ultisol by releasing low molecular weight organic acid, making phosphate to be bioavailable for plant usage. This will serve as biofertilizer in improving yield of crops in ferruginous ultisol and improve soil fertility.

microbiology↗