Resolving the heterogeneity of L1 DNA methylation reveals the epigenetic and transcriptional interplay between L1s and their integration sites.
Long interspersed element-1 (L1) retrotransposons play important roles in human disease and evolution. Their global activity is repressed by DNA methylation, but studying the regulation of individual copies has been difficult. Here, we combine short- and long-read sequencing to resolve the DNA methylation profiles of these repeated sequences in a panel of normal and cancer cells genome-wide at single-locus resolution. We unveil key principles underpinning L1 methylation heterogeneity among cell-types, families and integration sites. First, intronic L1 methylation is intimately associated with gene transcription. Conversely, L1s can influence the methylation status of the upstream region over short distances (300 bp). This phenomenon is accompanied by the binding of specific transcription factors, which drive the expression of L1 and chimeric transcripts. Finally, L1 hypomethylation alone is generally insufficient to trigger L1 expression due to redundant silencing pathways. Our results illuminate the epigenetic and transcriptional interplay between retrotransposons and their host genome. GRAPHICAL ABSTRACT O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=200 SRC="FIGDIR/small/522582v3_ufig1.gif" ALT="Figure 1"> View larger version (52K): org.highwire.dtl.DTLVardef@c78bbcorg.highwire.dtl.DTLVardef@a7112dorg.highwire.dtl.DTLVardef@58abf2org.highwire.dtl.DTLVardef@180d055_HPS_FORMAT_FIGEXP M_FIG C_FIG HIGHLIGHTSO_LIBs-ATLAS-seq profiles L1 position and methylation genome-wide C_LIO_LIL1 has a frequent but short-range (300 bp) influence on the DNA methylation status of the upstream sequence C_LIO_LIHypomethylated L1s are bound by tissue-specific transcription factors which drive L1 and chimeric transcripts synthesis C_LIO_LIL1 hypomethylation alone is insufficient to enable its transcription at most loci C_LI