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Ryckebusch, F.

Publications and source records attributed to Ryckebusch, F..

3 recordsLinked to original sources

Layers of immunity: Deconstructing the Drosophila effector response

The host innate immune response relies on the cooperation of multiple defence modules. In insects and other arthropods, which have only innate immune mechanisms, four main immune-specific modules have been described in the defence against microbial invaders: the Toll pathway, the Imd pathway, the melanization response, and phagocytosis by plasmatocytes. Our present understanding of their relative importance remains fragmented as their contribution to host defence has never been simultaneously assessed across a large panel of pathogens. Here, we have taken advantage of newly-described immune mutants in a controlled genetic background to systematically delete these four immune modules individually, in pairs, or even all four simultaneously. Surprisingly, immune deficient flies simultaneously deficient in all four immune modules are viable, homozygous fertile, and display no overt morphological defects, suggesting these immune mechanisms are not strictly required for organismal development. With this new set of lines, we assessed the contribution of each module individually and collectively to host defense against five viruses, three fungi, eight Gram-positive bacteria, and eight Gram-negative bacteria. Our findings show that these four modules largely function independently and additively in host defense, although synergistic effects can occur for select pairs of modules. Our study confirmed the importance of the Imd pathway against Gram-negative bacteria and the Toll pathway against Gram-positive bacteria and fungi, largely via the induction of effectors such as antimicrobial peptides (AMPs) and Bomanins (Bom), but also reveals the important role of melanization against viruses, and the contribution of phagocytosis against various germs. Additionally, by examining microbial load kinetics in different mutants, we provide insight into how these modules contribute to tolerance or resistance against specific microbes. Our study provides insights into the architecture of the Drosophila immune system, revealing differential requirements of immune modules according to each pathogen. The set of immune deficient lines provided here offer tools to better assess the role of these immune modules in host defense.

immunology↗

Alfalfa leaf curl virus is efficiently acquired by its aphid vector Aphis craccivora but inefficiently transmitted

Alfalfa leaf curl virus (ALCV) is the first geminivirus for which an aphid transmission was reported. Transmission by Aphis craccivora was determined previously to be highly specific and circulative. Using various complementary techniques, the transmission journey of ALCV was monitored from its uptake in an infected plant tissue up to the head of its vector. ALCV was shown to be restricted in the phloem using fluorescent in situ hybridization (FISH) and electropenetrography (EPG) monitoring of virus acquisition. Furthermore, the virus is heterogeneously distributed in the phloem as revealed by FISH and qPCR quantification of the viral DNA acquired by aphids monitored by EPG. In spite of the efficient ingestion of viral DNA, about 106 in a 15-hour feeding on ALCV infected plants, the individual transmission rate was at a maximum of 12%. Transmission success was related to a critical viral accumulation, around 1.6x107 viral DNA copies per insect, a threshold that needs generally more than 48 hours to be reached. Moreover, whereas the amount of acquired virus does not decrease over time in the whole aphid body, it decreased in hemolymph and heads. ALCV was not detected in progenies of viruliferous aphids and had no effect on aphid fitness. Compared to geminiviruses transmitted by whiteflies or leafhoppers or to luteovirus transmitted by aphids, the transmission efficiency of ALCV by A. craccivora is low. This result is discussed in relation to the aphid vector of this geminivirus and the agroecological features of alfalfa, a hardy perennial host plant.

ecology↗

Alfalfa leaf curl virus is transmitted by Aphis craccivorain a highly specific circulative manner

Two members of the genus Capulavirus (Geminiviridae) are transmitted by aphids including Alfalfa leaf curl virus (ALCV) transmitted by Aphis craccivora. The capulavirus Euphorbia caput-medusae latent virus was shown here to be transmitted also by A. craccivora, using the population EuphorbiaSA. ALCV was transmissible by several A. craccivora populations including Robinia, but not EuphorbiaSA, reflecting a high transmission specificity. ALCV accumulation and localization were analyzed in whole insects, midguts, hemolymphs, and heads of aphids from both populations and from the non-vector species Acyrthosiphon pisum. A 6-day persistence was observed in A. craccivora populations but not in A. pisum. Vector and non-vector A. craccivora populations could be distinguished by contrasted virus accumulations and midgut intracellular localization. Results confirm that ALCV is transmitted according to a circulative-persistent mode, and are consistent with a gut barrier to the transmission of ALCV in A. pisum and a primary salivary gland barrier in A. craccivora.

ecology↗