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Rybkin, I.

Publications and source records attributed to Rybkin, I..

2 recordsLinked to original sources

Strain-dependent selective antimicrobial action of cationic polyelectrolytes on Gram-negative bacteria

Although electrostatic modification of bacterial surfaces using polyelectrolytes (PEs) is a convenient and versatile tool for biotechnological processes, the ambiguities in toxicity of PEs between various bacteria and the insufficient understanding of the mechanism of action of cationic PEs and their nano-thick shells formed around the bacteria create a bottleneck of the approach. Here, we show how the viability of two bacterial strains, Escherichia coli and Pseudomonas stutzeri, both from the Gram-negative group differs, when the cells are exposed to cationic PEs under different conditions. Although the cell wall architecture of the strains should be structurally similar, we found that the viability of E. coli was not affected by the electrostatic deposition of polyethyleneimine (PEI) or poly(allylamine) hydrochloride (PAH), whereas for P. stutzeri the deposition resulted in high death rates. The cells of E. coli proved to be suitable templates for Layer-by-Layer (LbL) modification, while in P. stutzeri a modified protocol with mild conditions had to be used to ensure the viability of the cells. Super resolution stimulated emission depletion (STED) microscopy allowed us to clearly visualize that after PE deposition onto the surface of the cells, the PEs could penetrate inside the cells of P. stutzeri, while forming a capsule around E. coli as expected. Therefore, this knowledge will help us select the most appropriate combinations of strains and PEs, for biotechnological processes or biomedical application, preventing unwanted toxicity.

microbiology↗

Isolation, identification and selection of bacteria with the proof-of-concept for bioaugmentation of whitewater from woodfree paper mills

When whitewater circuits in the paper industry are closed, organic compounds accumulate and cause adverse production problems, such as the formation of slime and pitch. Since wood-free whitewater is usually a mixture of additives for paper production and an efficient cost-effective purification technology for their removal is lacking, the aim of our study was to find an effective bio-based strategy for whitewater treatment using a selection of indigenous bacterial isolates. We first obtained a large collection of bacterial isolates and then tested them individually for their ability to degrade the organic additives used in papermaking, i.e. carbohydrates, resin acids, alkyl ketene dimers, polyvinyl alcohol, latex, and azo and fluorescent dyes. Out of the 355 bacterial isolates, we selected a combination of four strains (Xanthomonadales bacterium sp. CST37-CF, Sphingomonas sp. BLA14-CF, Cellulosimicrobium sp. AKD4-BF and Aeromonas sp. RES19-BTP), which cover the entire spectrum of the tested organic additives. A proof-of-concept study in pilot scale was then performed by immobilizing the cells of our artificial bacterial consortium in a 33-liter tubular flow-through reactor with a retention time of <15 h. The combination of the four native strains enabled an 88% reduction in COD of whitewater even after 21 days. Additionally, we show that the bio-based whitewater treatment surpasses photolysis and photocatalysis. Graphical abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=77 SRC="FIGDIR/small/398669v2_ufig1.gif" ALT="Figure 1"> View larger version (30K): org.highwire.dtl.DTLVardef@9dd48eorg.highwire.dtl.DTLVardef@1edd8cdorg.highwire.dtl.DTLVardef@347eccorg.highwire.dtl.DTLVardef@708b63_HPS_FORMAT_FIGEXP M_FIG C_FIG HighlightsO_LIA strategy for selecting a consortium of indigenous bacteria with a high potential for bioaugmentation of wood-free whitewater is presented. C_LIO_LIStudy of bacterial degradation of eight chemically diverse substances used in paper production is presented. Methods for the selection of bacteria suitable for industrial application were developed. C_LIO_LIThe constructed artificial bacterial consortium consisted of strains belonging to genera Xanthomonadales bacterium, Sphingomonas, Aeromonas and Cellulosimicrobium. C_LIO_LIThe proof of concept of the industrial application, consisting of a 33-L-column filled with the immobilized artificial consortium, was an 88% decrease in COD of the whitewater effluent. C_LI

bioengineering↗