Biology subjects

Roy Chowdhury, M.

Publications and source records attributed to Roy Chowdhury, M..

3 recordsLinked to original sources

bioRxiv2026

Differential signaling by two Plasmodium sporozoite adhesins mediates transmission of malaria parasites

Malaria parasites are transmitted in the form of Plasmodium sporozoites, highly motile cells that form in oocysts at the mosquito midgut and invade salivary glands. After transmission to the vertebrate host, sporozoites migrate in the skin and liver to enter and differentiate in hepatocytes. Sporozoite migration and invasion are mediated by surface adhesins which link the extracellular substrate to the actin-myosin motor, providing the force for gliding motility. Here we show that two adhesins, TRAP and TRP1, use different extracellular domains and distinct cytoplasmic signaling to mediate salivary gland invasion and gliding motility. The extracellular thrombospondin repeat (TSR) of TRP1 but not the TSR of TRAP is essential for gliding and transmission and the TSR of TRAP can only partially rescue TSR function in TRP1. Furthermore, the cytoplasmic domain of TRAP cannot replace the equivalent domain of TRP1, which is essential for gliding and salivary gland invasion. This suggests that sporozoites integrate signals through multiple essential outside-in signaling pathways to achieve migration and invasion.

cell biology↗

bioRxiv2024

Elucidating role of long non-coding RNAs of Tamarindus indica Linn. in post-transcriptional gene regulation.

Tamarindus indica, commonly known as tamarind, is a rich source of carbohydrates, proteins, lipids, fatty acids, vitamins, minerals and different bioactive compounds. It is well established that long non-coding RNAs (lncRNAs) play important role in transcriptional, post-transcriptional and epigenetic regulation. In spite of the tamarind genome information available, handful of studies have been done on its non-coding genome. In this study, 320 tamarind lncRNAs have been predicted by computational methods. Along with the experimental validation of seven randomly chosen lncRNAs, functional analysis of the predicted lncRNAs along with their targets elucidated their roles in various biological pathways. Sequence analysis of these predicted lncRNAs reveals the presence of different motifs and TRs. Our analysis provides information about the non-coding genome of tamarind and their involvement in gene regulation, which may be used to gain more knowledge about the medicinal properties of tamarind.

plant biology↗

bioRxiv2018

Elucidating the functional role of predicted miRNAs in post-transcriptional gene regulation along with symbiosis in Medicago truncatula

Non-coding RNAs (ncRNAs) are found to be important regulator of gene expression because of their ability to modulate post-transcriptional processes. microRNAs are small ncRNAs which inhibit translational and post-transcriptional processes whereas long ncRNAs are found to regulate both transcriptional and post-transcriptional gene expression. Medicago truncatula is a well-known model plant for studying legume biology and is also used as a forage crop. In spite of its importance in nitrogen fixation and soil fertility improvement, little information is available about Medicago ncRNAs that play important role in symbiosis. To understand the role of Medicago ncRNAs in symbiosis and regulation of transcription factors, we have identified novel miRNAs and tried to establish an interaction model with their targets. 149 novel miRNAs are predicted along with their 770 target proteins. We have shown that 51 of these novel miRNAs are targeting 282 lncRNAs. We have analyzed the interactions between miRNAs and their target mRNAs as well as their targets on lncRNAs. Role of Medicago miRNAs in the regulation of various transcription factors were also elucidated. Knowledge gained from this study will have a positive impact on the nitrogen fixing ability of this important model plant, which in turn will improve the soil fertility.

genomics↗