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Rouquie, D.

Publications and source records attributed to Rouquie, D..

5 recordsLinked to original sources

Model Validation Protocols for Machine Learning in Small Molecule Drug Discovery

Machine learning (ML) models for molecular property prediction are increasingly deployed in drug discovery, yet their adoption in real-world scenarios requires an understanding of the conditions in which a model succeeds or fails. While standardized benchmarks are powerful instruments to measure and unlock progress in ML research, they should not be blindly treated as the end goal. Especially static and retrospective benchmarks, in which no true unknown test set is employed, limit our ability to robustly validate a model's performance. Building on the collective expertise of a cross-industry consortium, we present a model validation framework consisting of five recommendations that would enable the community to move beyond aggregate metrics toward understanding where and why molecular property prediction models fail. We connect evaluation choices to real-world applications and case studies encountered in pharmaceutical research. The framework proposes splitting strategies that mimic realistic distribution shifts and expose common failure modes. We apply the recommended framework to a recently released dataset of absorption, distribution, metabolism, and excretion (ADME) properties. Across two complementary model algorithms, our case studies reveal four distinct failure modes (extrapolation, interpolation, representation, and evaluation), showing that model errors arise not only from distribution shift but also from limitations in molecular representations. Our results show that commonly used evaluation protocols can significantly overestimate performance and may not detect important model failure modes. All software and data are released via https://github.com/srijitseal/polaris.

bioinformatics↗

Large-scale experimental validation of phenotype-guided generative AI for de novo drug discovery

Generative AI is increasingly used to design drugs with desired physicochemical and biological properties. We have previously used the cGAN algorithm trained on cell painting data to generate molecules targeting 10 molecular cancer targets. Here, we provide experimental evidence of the cGAN performance in designing chemical compounds with desired biological activity. We chose a subset of the AI-generated structures for chemical synthesis and tested their activity in a cell painting and a transcriptomic assay. In summary, 88% of the compounds had a pronounced impact on overall cellular morphology, and 37% of the compounds significantly affected transcriptomic pathways associated with the intended target. In a cellular activity assay for an exemplary target, TP53, 6 out of 9 compounds showed potential to modulate TP53 activity. Overall, we show the value of conditioning a generative model on phenotypic readouts for hit discovery of new small molecule drug candidates and pave the way toward AI-guided chemical safety by design.

pharmacology and toxicology↗

Cell Painting for cytotoxicity and mode-of-action analysis in primary human hepatocytes

High-throughput, human-relevant approaches for predicting chemical toxicity are urgently needed for better decision-making in human health. Here, we apply image-based profiling (the Cell Painting assay) and two cytotoxicity assays (metabolic and membrane damage readouts) to primary human hepatocytes after exposure to eight concentrations of 1085 compounds that include pharmaceuticals, pesticides, and industrial chemicals with known liver toxicity-related outcomes. Three computational methods (CellProfiler, a Cell Painting-specific convolutional neural network, and a pretrained vision transformer) were compared to extract morphology features from single cells or entire images. We used these morphology features to predict activity in the measured cytotoxicity assays, as well as in 412 curated ToxCast assays that span cytotoxicity, cell-based, and cell-free categories. We found that the morphological profiles detect compound bioactivity at lower concentrations than standard cytotoxicity assays. In supervised analyses, they predict cytotoxicity and targeted cell-based assay readouts, but not cell-free assay readouts. We also found that the various feature extraction methods performed relatively similarly and that filtering out non-bioactive or cytotoxic concentrations did not boost supervised assay prediction performance for any assay endpoint category, although it did have a large influence on unsupervised cluster analysis. We envision that image-based profiling could serve as a key component of modern safety assessment.

pharmacology and toxicology↗

Cell morphology and gene expression: tracking changes and complementarity across time and cell lines

Effective drug discovery relies on combining target knowledge with functional assays and multi-omics data to understand chemicals molecular actions. However, the relationship between cell morphology and gene expression over time and across cell lines remains unclear. To explore this, we analyzed Cell Painting and L1000 data for 106 compounds across three cell lines from osteoblast, lung, and breast tumors (U2OS, A549, and MCF7) at three time points (6h, 24h, 48h) using a 10{micro}M concentration. We found significant time and cell line effects in Cell Painting data, with less pronounced effects in transcriptomics. Using Weighted Gene Co-expression Network Analysis (WGCNA) and enrichment analysis, we identified connections between cell morphology and gene deregulation for chemicals with similar biological effects (e.g., HDAC and CDK inhibitors). These findings suggest that while Cell Painting shows distinct patterns, both technologies offer complementary insights into compound-induced cellular changes, enhancing drug discovery and chemical risk assessment.

bioinformatics↗

Cell Painting morphological profiles can complement QSAR models for rat acute oral toxicity prediction

Early de-risking decisions in the development of new chemical compounds, enable the identification of novel chemical candidates with improved safety profiles. In vivo studies are traditionally conducted in the early assessment of acute oral toxicity of crop protection products to avoid compounds which are considered "very acutely toxic", with an in vivo Lethal Dose of 50% (LD50) [≤] 60 mg/kg bodyweight. Those studies are lengthy, costly, and raise ethical concerns, catalyzing the use of non-animal alternatives. The objective of our analysis was to assess the predictive efficacy of read-across approaches for acute oral toxicity in rats, comparing the use of chemical structure information, in vitro biological data derived from the Cell Painting profiling assay on U2OS cells or the combination of both. Our findings indicate that the classification of compounds as very acute oral toxic (LD50 [≤] 60 mg/kg) or not is possible using a read-across approach, with chemical structure information, morphological profiles, or a combination of both. When classifying compounds structurally similar to those in the training set, chemical structure was more predictive (balanced accuracy of 0.82). Conversely, when the compounds to be classified were structurally different from those in the training set, the morphological profiles were more predictive (balanced accuracy of 0.72). Combining the two models allowed for the classification of compounds structurally similar to those in the training set, to slightly improve the predictions (balanced accuracy of 0.85). O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=102 SRC="FIGDIR/small/591123v2_ufig1.gif" ALT="Figure 1"> View larger version (29K): org.highwire.dtl.DTLVardef@d7d4fcorg.highwire.dtl.DTLVardef@1e00a92org.highwire.dtl.DTLVardef@1d60fccorg.highwire.dtl.DTLVardef@a72961_HPS_FORMAT_FIGEXP M_FIG For Table of Contents Only C_FIG

pharmacology and toxicology↗