Biology subjects

Roshan, S. A.

Publications and source records attributed to Roshan, S. A..

3 recordsLinked to original sources

bioRxiv2023

Short-term intermittent hypoxia therapy promotes gliogenesis in a rat model of middle cerebral artery occlusion (MCAO) stroke

AimAfter focal cerebral ischemia, intermittent hypoxia therapy (IHT) could be used as a non-invasive method to stabilize and stimulate neurogenesis in the innate stem cell niche in the brain, and disrupt the glial scar around the infarct to increase neuroblast migration in the striatal infarct area. MethodsWe induced focal cerebral ischemia in Wistar albino rats using the MCAo model. A week later, animals were subjected to intermittent hypoxia (12%O2, 4hr/day) for a period of 14 days. Post-treatment analysis of functional recovery and cellular regeneration was done using immunofluorescence analysis of multiple neuronal cell markers including Doublecortin (DCX), Nestin, and Vimentin among others. ResultsObservations of GFAP-positive cells revealed that IH treatment facilitates gliogenesis in the infarct striatal region of a rat model of MCAo stroke. The percentage of DCX and GFAP double-positive cells was increased in the IH-treated group. Also, there was a significant difference in the morphology of vimentin-positive cells and microglia cells between the stroke groups. ConclusionThese outcomes suggest that exposure of MCAo stroke-affected rats to intermittent hypoxia results in an increase in migrated neuroblasts resulting in a subsequent altered glial scar integrity in the infarct region, thus suggesting an alternative non-invasive method against the common stem cell transplant techniques, to increase endogenous neuroblasts in the infarct area after stroke.

neuroscience↗

bioRxiv2023

Design and Evaluation of an Affordable Hypoxic Chamber with Comprehensive Environmental Control for Research Applications

PurposeIn-vivo Hypoxia chamber is sought after for research on sleep apnea, among others. Recently hypoxia chambers are utilized to create intermittent hypoxia, thereby utilizing it as a treatment strategy. The commonly used Invivo hypoxic chambers only monitor oxygen levels and fail to read up on vital factors like CO2 buildup, and humidity among others. In addition, these devices are expensive and make them unaffordable for many labs. MethodsHere we report a lab-designed and assembled Arduino microcontroller-based hypoxic chamber with automatic maintenance of O2 level, CO2 level, and Humidity. The entire 650$ setup is inclusive of an Arduino module with sensors for oxygen, CO2, humidity, pressure, and temperature. A detailed software program was also written to help efficiently carry out the parameters set out for use. ResultsThe equipment is capable of automatically regulating the inner environment based on the set parameters. The combined cluster of regulators, efficiently controlled the oxygen levels, CO2 levels, and humidity levels within the experimental parameters. ConclusionThis equipment thus acts as one of the easily reproducible simple assembly units with the integration of complex parameters, thus monitoring and controlling the inner environment with high precision.

bioengineering↗

bioRxiv2021

Altered neurogenic pathways in experimental rat models of ischemic cerebral stroke: A transcriptome based meta-analysis

1)ObjectivesPromoting neurogenesis mediated recovery is one of the most sought after strategies in recovery after cerebral stroke. In this paper we elucidate how neurogenesis related genes are altered in the early stroke environment, to hint at potential pathways for therapeutic recovery. Materials and MethodsAround 97 microarray datasets derived from stroke affected rat brains were collected from NCBI-GEO. Datasets were normalized and subjected to meta-analysis in Network Analyst to identify differentially expressed genes. Gene enrichment analyses were carried out using GSEA, and WebGestalt, and results were visualized using Cytoscape Enrichment mapping. ResultsNearly 939 differentially expressing genes were identified in the cerebral stroke group. Among them 30 neurogenesis related genes were identified through enrichment mapping analysis, and 35 genes through Protein- Protein Interaction analysis. Highest upregulated neurogenesis genes were found to be TSPO, GFAP, VIM, and TGFB1. Highest Downregulated neurogenesis genes were found to be THY1, NR1D1, CDK5, STX1B, and NOG. ConclusionsThrough this study, we have identified that during the acute time frame after stroke, majority of the neurogenesis genes related to neural proliferation and neural differentiation are downregulated, while the majority of the genes related to neuronal migration were upregulated. Single or combined therapeutic approach against the identified dysregulated genes could greatly aid neural restoration and functional recovery during the postischemic stage.

neuroscience↗