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Biology subjects

Rosas, N.

Publications and source records attributed to Rosas, N..

2 recordsLinked to original sources

Presence of Aedes aegypti in a high-altitude area in Bolivia

Aedes aegypti is currently widespread in Bolivia. Although these mosquitoes commonly inhabit tropical and subtropical areas at low altitudes, recent studies found them up to 2,500 meters above sea levels (m.a.s.l.). The studys objective was to identify the presence of the Ae. aegypti mosquito in urban areas with altitudes around 2,900 m.a.s.l. in Sucre, Bolivia. For this, ovitraps were installed at 12 points, and a morphological and taxonomic analysis was performed. The presence of mature and immature forms was confirmed in 3 of the 12 sampling points. Entomological indexes reported: House=13.64, Breteau= 27.27, dry containers= 43.55, containers with water =56.45. Reproductive populations of Ae. aegypti was confirmed to more than 2,900 m.a.s.l. in an urban area with a high migratory flow area. It increases the risk for autochthonous cases of Dengue Disease in the short term. Prevention and control strategies must be intensified to reduce the transmission of mosquito-borne diseases.

evolutionary biology↗

Treatment history shapes the evolution of complex carbapenem-resistant phenotypes in Klebsiella spp.

Antibiotic resistance is driven by selection, but how a bacterial strains evolutionary history shapes drug-resistance remains an open question. Here we reconstruct the genetic and evolutionary mechanisms of carbapenem resistance in a clinical isolate of Klebsiella. A combination of short and long read sequencing, machine learning, genetic and enzymatic analyses established that this carbapenem-resistant strain carries no carbapenemase-encoding genes. Genetic reconstruction of the resistance phenotype confirmed that two distinct genetic loci are necessary for the strain to acquire carbapenem resistance. Experimental evolution of the carbapenem-resistant strains in growth conditions without the antibiotic revealed that both loci confer a significant cost, and are readily lost by de novo mutation resulting in the rapid evolution of a carbapenem-sensitive phenotype. Thus, historical contingency - a patients treatment history - can shape the evolution of antibiotic resistance and suggests that the strategic combinations of antibiotics could direct the evolution of low-fitness, drug-resistant genotypes.

microbiology↗